A headspace-SPME-MS method for monitoring rapeseed oil autoxidation
A headspace-SPME-MS method for monitoring rapeseed oil autoxidation
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DOI：
10.1007/s11746-007-1072-2
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发表时间：
2007-06-01
影响因子：
2
通讯作者：
Wasowicz, E.
中科院分区：
文献类型：
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作者：
Jelen, H. H.;Mildner-Szkudlarz, S.;Wasowicz, E.
使用顶空 SPME-MS 分析菜籽油中的挥发性化合物，该菜籽油经过加速储存测试，包括在 60 摄氏度下储存 0-12 天。将 SPME-MS 数据与固相微萃取-气相色谱/质谱 (SPME-GUMS) 获得的数据进行比较。 SPME-GC/MS 方法可检测 37 种挥发性化合物，其中 28 种得到鉴定。主要是己醛、2,4-庚二烯醛、2-庚烯醛和1-戊烯-3-醇。在 SPME-MS 中未分离挥发性化合物 - 获得反映挥发物总量的单峰。该峰中特征离子丰度的增加可用于检测菜籽油自氧化特征的化合物。这些化合物（及其特征离子）为己醛（m/z 56）、1-戊烯-3-醇和1-辛烯-3-醇（m/z 57）、2-戊烯醛和2-庚烯醛（m/z 83和84）以及2,4-庚二烯醛（离子m/z 81和1·10）。 SPME-MS 峰面积与过氧化值相关（0.9779），与 Totox 相关（0.9841）。对模型菜籽油中脂肪酸挥发性氧化产物的主成分分析 (PCA) 表明，SPME-MS 能够区分含有浓度为 0.2 的己醛的样品。 0.4 0.6。 0.8 和 1.0 mg/L，与其他化合物成比例。此外，使用SPME-MS-PCA区分在60℃下储存0、2、4、6、81、10和12天的样品。 PCA 显示了 SPME-MS 和感官分析获得的数据聚类的相似性。
Headspace SPME-MS was used to analyze volatile compounds from rapeseed oil subjected to an accelerated storage test consisting of 0-12 days of storage at 60 degrees C. The SPME-MS data was compared with the data obtained by solid phase microextraction-gas chromatography/mass spectrometry (SPME-GUMS). The SPME-GC/MS method allowed detection of 37 volatile compounds, of which 28 were identified. Predominant ones were hexanal, 2,4-heptadienal, 2-heptenal and 1-pentene-3-ol. Volatile compounds were not separated in SPME-MS-a single peak reflecting the total amount of volatiles was obtained. An increase in the abundance of characteristic ions in this peak could be used to detect of compounds characteristic for rapeseed oil autoxidation. These compounds (with their characteristic ions) were hexanal (m/z 56), 1-pentene-3-ol and 1-octene-3-ol (m/z 57), 2-pentenal and 2-heptenal (m/z 83 and 84), and 2,4-heptadienal (ions m/z 81 and 1 10). The SPME-MS peak area was correlated with peroxide value at 0.9779 and with Totox at 0.9841. Principal component analysis (PCA) of fatty acid volatile oxidation products from a model rapeseed oil indicated that SPME-MS was able to differentiate samples containing hexanal at a concentration of 0.2. 0.4 0.6. 0.8 and 1.0 mg/L with proportional amounts of other compounds. Further, samples that were subjected to 0, 2 4 6, 81 10 and 12 days of storage at 60 degrees C were differentiated using SPME-MS-PCA. PCA showed similarities in clustering of the data obtained by SPME-MS and sensory analysis.