Rhinovirus-induced modulation of gene expression in bronchial epithelial cells from subjects with asthma.
Rhinovirus-induced modulation of gene expression in bronchial epithelial cells from subjects with asthma.
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鼻病毒(RV)感染会引发哮喘加重。对来自正常和哮喘供者的RV1A感染的原代支气管上皮细胞进行全基因组表达分析，以确定哮喘是否与RV诱导的基因表达的独特模式有关。来自正常捐赠者和哮喘捐赠者的细胞中的病毒复制率相似。总的来说，RV下调了975个基因，上调了69个基因。从芯片产生的转录图谱的比较，并经定量逆转录聚合酶链式反应和聚类分析证实，显示了哮喘细胞中一些上调和下调的基因，涉及免疫反应(IL1B，IL1F9，IL24，IFI44)和气道重塑(LOXL2，MMP10，FN1)。值得注意的是，RV感染细胞中与哮喘相关的大部分差异也存在于感染前的细胞中。这些发现表明，RV诱导的正常和轻度哮喘患者细胞基因表达谱的差异可能会影响RV的急性炎症反应以及随后的呼吸道修复和重塑。
Rhinovirus (RV) infections trigger asthma exacerbations. Genome-wide expression analysis of RV1A-infected primary bronchial epithelial cells from normal and asthmatic donors was performed to determine whether asthma is associated with a unique pattern of RV-induced gene expression. Virus replication rates were similar in cells from normal and asthmatic donors. Overall, RV downregulated 975 and upregulated 69 genes. Comparisons of transcriptional profiles generated from microarrays and confirmed by quantitative reverse transcription PCR and cluster analysis showed some up- and downregulated genes in asthma cells involved in immune responses (IL1B, IL1F9, IL24, IFI44) and airway remodeling (LOXL2, MMP10, FN1). Notably, most of the asthma-related differences in RV-infected cells were also present in the cells before infection. These findings suggest that differences in RV-induced gene expression profiles of cells from normal and mild asthmatic subjects could affect the acute inflammatory response to RV and subsequent airway repair and remodeling.
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影响因子：
5.6
作者：
Adiseshaiah, Pavan;Vaz, Michelle;Machireddy, Narsa;Kalvakolanu, Dhananjaya V.;Reddy, Sekhar P.
通讯作者：
Reddy, Sekhar P.
影响因子：
4.4
作者：
Ingram, Jennifer L.;Antao-Menezes, Aurita;Bonner, James C.
通讯作者：
Bonner, James C.
DOI：
10.1016/j.jaci.2008.07.007
发表时间：
2008-09
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Goleva E;Hauk PJ;Hall CF;Liu AH;Riches DW;Martin RJ;Leung DY
通讯作者：
Leung DY
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.1164/rccm.200404-532oc
发表时间：
2005-03-15
影响因子：
24.7
作者：
Lilly, CM;Tateno, H;Sonna, LA
通讯作者：
Sonna, LA