De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite
De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite
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DOI：
10.1186/s12864-015-2254-1
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发表时间：
2015-12-09
期刊：
影响因子：
4.4
通讯作者：
Hoffman, Joseph Ivan
中科院分区：
文献类型：
--
作者：
Pauli, Martina;Chakarov, Nayden;Hoffman, Joseph Ivan
背景资料：非模式物种的研究对于理解自然生态条件下表型变异的分子过程具有重要意义。秃鹰（学名：Buteo buteo; Aves：Accipitriformes）是一种分布广泛的欧亚猛禽，有三种不同的羽毛形态，在几个与健康相关的特征上不同，包括寄生虫感染。为了提供一个基因组资源的羽毛多态性鸟类一般和寻找候选基因的健身，我们产生了一个转录组从一个单一的死亡秃鹰标本加上容易获得的，微创的样品从活chicks.Results：我们不仅从头组装一个接近完整的秃鹰转录组，但也获得了一个显着的分数的转录组的疟疾样寄生虫，Leucocytozoon buteonis。通过鉴定在浅色腹侧和深色背侧羽毛中差异表达的黑素生成相关转录物，但也在身体的其他区域表达，我们还鉴定了一组可能与变体之间的适应性差异相关的候选基因。这些包括几个免疫相关的基因，提供了一个合理的黑化和寄生虫负载之间的联系。这些基因的一个子集的qPCR分析显示腹侧和背侧的羽毛和morph.Conclusion的额外效果之间的显着差异：这种新的资源提供了初步的见解基因，可能参与健身之间的差异秃鹰的颜色变体，并应促进未来的研究猛禽和他们的疟疾样寄生虫。
Background: Studies of non-model species are important for understanding the molecular processes underpinning phenotypic variation under natural ecological conditions. The common buzzard (Buteo buteo; Aves: Accipitriformes) is a widespread and common Eurasian raptor with three distinct plumage morphs that differ in several fitness-related traits, including parasite infestation. To provide a genomic resource for plumage polymorphic birds in general and to search for candidate genes relating to fitness, we generated a transcriptome from a single dead buzzard specimen plus easily accessible, minimally invasive samples from live chicks.Results: We not only de novo assembled a near-complete buzzard transcriptome, but also obtained a significant fraction of the transcriptome of its malaria-like parasite, Leucocytozoon buteonis. By identifying melanogenesis-related transcripts that are differentially expressed in light ventral and dark dorsal feathers, but which are also expressed in other regions of the body, we also identified a suite of candidate genes that could be associated with fitness differences among the morphs. These include several immune-related genes, providing a plausible link between melanisation and parasite load. qPCR analysis of a subset of these genes revealed significant differences between ventral and dorsal feathers and an additional effect of morph.Conclusion: This new resource provides preliminary insights into genes that could be involved in fitness differences between the buzzard colour morphs, and should facilitate future studies of raptors and their malaria-like parasites.