A Mouse Model of Acute Cartilage Injury and Repair.
A Mouse Model of Acute Cartilage Injury and Repair.
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急性软骨损伤和修复的小鼠模型。
DOI：
10.1007/978-1-0716-2839-3_24
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Thorup AS
中科院分区：
文献类型：
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作者：
Thorup AS
软骨缺陷很常见并且会致残。软骨修复药理学方法的发展需要体内模型的可用性，这些模型能够适应功能的获得和丧失，并且最好能够进行基因改造。在本章中，我们描述了一种诱导全层软骨缺损的方法，该缺损在年轻的 DBA/1 小鼠中可以自发愈合，但在同龄的 C57BL/6 小鼠或老年 DBA/1 小鼠中则无法愈合。该模型（或变体）已用于遗传筛选，以识别与修复能力相关的基因，研究参与软骨修复的干细胞，以及研究参与修复机制的分子的功能。
Chondral defects are common and disabling. The development of pharmacological approaches for cartilage repair requires the availability of in vivo models which are amenable for gain and loss of function and ideally to genetic modification. In this chapter, we describe a method to induce full-thickness cartilage defects which, in young DBA/1 mice, heal spontaneously, but fail to heal in C57BL/6 mice of the same age or in aged DBA/1 mice. This model (or variants) has been used for genetic screenings to identify genes associated to repair capacity, to study stem cells involved in cartilage repair, and to study the function of molecules involved in repair mechanisms.