NUCLEOTIDE-SEQUENCE OF THE PROKARYOTIC MOBILE GENETIC ELEMENT IS30
NUCLEOTIDE-SEQUENCE OF THE PROKARYOTIC MOBILE GENETIC ELEMENT IS30
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DOI：
10.1002/j.1460-2075.1984.tb02104.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
ARBER, W
中科院分区：
文献类型：
--
作者：
DALRYMPLE, B;CASPERS, P;ARBER, W
移动的遗传元件IS 30是大肠杆菌K12的常驻基因，其完整的核苷酸序列长1221 bp。一个大的开放阅读框架，前面可能是转录和翻译控制信号，可以编码一个383个氨基酸的基本蛋白质，可能作为转座酶的功能。在相对链上不存在大的框内开放阅读框。26-bp长的末端反向重复与噬菌体Mu基因组的c-末端和盐生盐杆菌插入元件ISH 50的末端反向重复具有一些序列同源性。IS 30序列与任何其他已测序的原核插入序列没有显著的同源性。
The complete nucleotide sequence of the mobile genetic element IS30, a resident of Escherichia coli K12, is 1221 bp [base-pairs] long. A large open reading frame, preceded by possible transcription and translation control signals, could encode a basic protein of 383 amino acids which might presumably function as transposase. No large in-frame open reading frame is present on the opposite strand The 26-bp-long terminal inverted repeats have some sequence homology with the c-end of the phage Mu genome and with the terminal inverted repeats of the Halobacterium halobium insertion element ISH50. The IS30 sequence has no significant homology with any other sequenced prokaryotic insertion sequences.