Globin gene server: a prototype E-mail database server featuring extensive multiple alignments and data compilation for electronic genetic analysis.
Globin gene server: a prototype E-mail database server featuring extensive multiple alignments and data compilation for electronic genetic analysis.
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Globin 基因服务器：原型电子邮件数据库服务器，具有用于电子遗传分析的广泛多重比对和数据编译。
DOI：
10.1006/geno.1994.1275
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发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Miller,W
中科院分区：
文献类型：
--
作者：
Hardison,R;Chao,KM;Schwartz,S;Stojanovic,N;Ganetsky,M;Miller,W
几乎整个β-样珠蛋白基因簇的序列已经从几种哺乳动物中确定，并且许多调节区域已经通过诱变、功能测定和核蛋白结合研究进行了分析。需要以易于访问和可用的方式编译这些非常大量的序列和功能数据，以优化数据分析、假设检验和模型构建。我们报告了一个珠蛋白基因服务器，它将在全面实施时以不断更新的方式提供这项服务。服务器有两个主要功能。第一个（目前可用）提供了一个注释的DNA序列在整个基因簇从所有物种的代表分析的多重比对。第二个汇编数据的功能和蛋白质结合分析整个基因簇。使用来自五个物种的β-珠蛋白基因的比对的5'侧翼区的这种编译的原型显示了以下的实例：（1）已经证明功能的良好保守的区域，包括功能数据明显冲突的情况，（2）提出的不很保守的功能区域，和（3）目前没有指定功能的保守区域。这样的电子遗传分析导致许多容易检验的假设，这些假设在没有多重比对和编译的情况下并不立即明显。该服务器可通过电子邮件在计算机网络上访问，打印的结果可以通过请求获得的作者。这个原型将是一个有用的指南，为许多基因组位点开发类似的工具。
The sequence of virtually the entire cluster of β-like globin genes has been determined from several mammals, and many regulatory regions have been analyzed by mutagenesis, functional assays, and nuclear protein binding studies. This very large amount of sequence and functional data needs to be compiled in a readily accessible and usable manner to optimize data analysis, hypothesis testing, and model building. We report a Globin Gene Server that will provide this service in a constantly updated manner when fully implemented. The Server has two principal functions. The first (currently available) provides an annotated multiple alignment of the DNA sequences throughout the gene cluster from representatives of all species analyzed. The second compiles data on functional and protein binding assays throughout the gene cluster. A prototype of this compilation using the aligned 5' flanking region of β-globin genes from five species shows examples of (1) well-conserved regions that have demonstrated functions, including cases in which the functional data are in apparent conflict, (2) proposed functional regions that are not well conserved, and (3) conserved regions with no currently assigned function. Such an electronic genetic analysis leads to many readily testable hypotheses that were not immediately apparent without the multiple alignment and compilation. The Server is accessible via E-mail on computer networks, and printed results can be obtained by request to the authors. This prototype will be a helpful guide for developing similar tools for many genomic loci.