Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors.
Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors.
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DOI：
10.1007/978-1-0716-1956-8_4
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发表时间：
2022
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质谱流式细胞术又名飞行时间细胞术 (CyTOF)，是最近开发的几种多参数单细胞技术之一，旨在解决健康和患病组织内的细胞异质性。质谱细胞术是流式细胞术的改进，其中抗体用稳定的重金属同位素标记，并通过飞行时间质谱法进行读数。由于通道之间的溢出极小，质谱流式分析仪可以读取每个单细胞多达 60 个参数。至关重要的是，质谱流式细胞术可以识别在大量组织分析中丢失的少数细胞群。质谱流式细胞术在免疫细胞的研究中发挥了巨大作用。我们已将其用途扩展到检查分解的实体组织内的单细胞，特别是新鲜切除的输卵管卵巢高级浆液性肿瘤。在这里，我们详细介绍了旨在确保生成高质量单细胞数据集的协议。该方法可以进行修改以适应其他实体组织的研究。
Mass cytometry aka Cytometry by Time-Of-Flight (CyTOF) is one of several recently developed multiparametric single-cell technologies designed to address cellular heterogeneity within healthy and diseased tissue. Mass cytometry is an adaptation of flow cytometry in which antibodies are labeled with stable heavy metal isotopes and the readout is by time-of-flight mass spectrometry. With minimal spillover between channels, mass cytometry enables readouts of up to 60 parameters per single cell. Critically, mass cytometry can identify minority cell populations that are lost in bulk tissue analysis. Mass cytometry has been used to great effect for the study of immune cells. We have extended its use to examine single cells within disaggregated solid tissues, specifically freshly resected tubo-ovarian high-grade serous tumors. Here we detail our protocols designed to ensure the production of high-quality single-cell datasets. The methodology can be modified to accommodate the study of other solid tissues.