BIOFILM FORMATION BY CANDIDA SPECIES ON THE SURFACE OF CATHETER MATERIALS IN-VITRO
BIOFILM FORMATION BY CANDIDA SPECIES ON THE SURFACE OF CATHETER MATERIALS IN-VITRO
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DOI：
10.1128/iai.62.3.915-921.1994
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发表时间：
1994-03-01
影响因子：
3.1
通讯作者：
DOUGLAS, LJ
中科院分区：
文献类型：
--
作者：
HAWSER, SP;DOUGLAS, LJ
描述了用于研究在导管材料小圆盘表面上生长的念珠菌生物膜的模型系统。通过涉及四唑盐还原的比色测定或通过[H-3]亮氨酸掺入来定量测定生物膜的形成；两种方法都与生物膜干重具有良好的相关性（分别为 r = 0.997 和 0.945）。白色念珠菌生物膜在含有500 mM半乳糖或50 mM葡萄糖的培养基中的生长在48小时后达到最大值，然后下降；然而，低葡萄糖培养基中的细胞产量较低。 d. 15 种不同分离株生物膜形成的比较。白色念珠菌未能揭示与该组内致病性的任何相关性，但当测试不同的念珠菌属时，与致病性存在一定相关性。近平滑念珠菌（格拉斯哥）、拟热带念珠菌和光滑念珠菌的分离株均比致病性更强的白色念珠菌的生物膜生长显着减少（P < 0.001）。对各种导管材料的评估表明，与聚氯乙烯相比，乳胶或有机硅弹性体上白色念珠菌形成的生物膜略有增加（P < 0.05），但在聚氨酯或 100% 有机硅上则显着减少（P < 0.001）。扫描电镜显示，48小时后，白色念珠菌生物膜由酵母、芽管、假菌丝和菌丝组成的致密网络组成；在某些形态形式的表面上可以看到细胞外聚合材料。我们的模型系统是研究念珠菌生物膜的一种简单方便的方法，可用于测试抗真菌药物对生物膜细胞的功效。
A model system for studying Candida biofilms growing on the surface of small discs of catheter material is described. Biofilm formation was determined quantitatively by a colorimetric assay involving reduction of a tetrazolium salt or by [H-3]leucine incorporation; both methods gave excellent correlation with biofilm dry weight (r = 0.997 and 0.945, respectively). Growth of Candida albicans biofilms in medium containing 500 mM galactose or 50 mM glucose reached a maximum after 48 h and then declined; however, the cell yield was lower in low-glucose medium. Comparison of biofilm formation by 15 different isolates of d. albicans failed to reveal any correlation with pathogenicity within this group, but there was some correlation with pathogenicity when different Candida Species were tested. Isolates of C. parapsilosis (Glasgow), C. pseudotropicalis, and C. glabrata all gave significantly less biofilm growth (P < 0.001) than the more pathogenic C. albicans. Evaluation of various catheter materials showed that biofilm formation by C. albicans was slightly increased on latex or silicone elastomer (P < 0.05), compared with polyvinyl chloride, but substantially decreased on polyurethane or 100% silicone (P < 0.001). Scanning electron microscopy demonstrated that after 48 h, C. albicans biofilms consisted of a dense network of yeasts, germ tubes, pseudohyphae, and hyphae; extracellular polymeric material was visible on the surfaces of some of these morphological forms. Our model system is a simple and convenient method for studying Candida biofilms and could be used for testing the efficacy of antifungal agents against biofilm cells.