STIMULATORY EFFECTS OF ATRIAL NATRIURETIC FACTOR ON PHOSPHOINOSITIDE HYDROLYSIS IN CULTURED BOVINE AORTIC SMOOTH-MUSCLE CELLS
STIMULATORY EFFECTS OF ATRIAL NATRIURETIC FACTOR ON PHOSPHOINOSITIDE HYDROLYSIS IN CULTURED BOVINE AORTIC SMOOTH-MUSCLE CELLS
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DOI：
10.1016/0167-4889(89)90060-8
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发表时间：
1989-03-06
期刊：
影响因子：
--
通讯作者：
MURAD, F
中科院分区：
文献类型：
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作者：
HIRATA, M;CHANG, CH;MURAD, F
在培养的牛主动脉平滑肌细胞制备物中检测了心房钠尿因子（ANF）对磷酸肌醇水解的影响。在培养的牛主动脉平滑肌细胞的颗粒组分的匀浆中，ANF和心房肽素I增加肌醇磷酸的形成和ATP酶活性。鸟苷5 "[γ-氨基]显著增强了对肌醇磷酸的作用。硫代]三磷酸盐。两种心房肽也刺激了完整培养细胞中甘油二酯的形成。在这些实验中，心房肽I的效力比ANF强约10倍。这些研究表明，心房肽对磷酸肌醇水解具有刺激作用，这种作用是通过鸟嘌呤核苷酸调节蛋白介导的。心房肽I对GTP酶活性和肌醇磷酸积累的更大效力表明，ANF的非鸟苷酸环化酶偶联受体（ANF-R2）通过鸟嘌呤核苷酸调节蛋白介导ANF对磷酸肌醇水解的刺激作用。
The effects of atrial natriuretic factor (ANF) on phosphoinositide hydrolysis were examined in preparations of cultured bovine aortic smooth muscle cells. In homogenates of particulate fractions from cultured bovine aortic smooth muslce cells, ANF and atriopeptin I increased the formation of inositol phosphates and ATPase activity. The effects on inositol phosphates were markedly enhanced with guanosine 5''[.gamma.-thio]triphosphate. Both atrial peptides also stimulated the formation of diacylglycerol in intact cultured cells. In these experiments, atriopeptin I was about 10-fold more potent than ANF. These studies indicate that atrial peptides have stimulatory effects on phosphoinositide hydrolysis which are mediated through a guanine nucleotide regulatory protein. The greater potency of atriopeptin I on GTPase activity and the accumulation of inositol phosphates suggests that the nonguanylate cyclase-coupled receptor for ANF (ANF-R2) mediates the stimulatory effects of ANF on phosphoinositide hydrolysis through a guanine nucleotide regulatory protein.