Effect of glycerol treatment on transmitter release at the frog neuromuscular junction.
Effect of glycerol treatment on transmitter release at the frog neuromuscular junction.
复制标题
甘油处理对青蛙神经肌肉接头递质释放的影响。
DOI：
10.1016/0006-8993(93)90293-v
复制
发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Zengel,JE
中科院分区：
文献类型：
--
作者：
Sosa,MA;Zengel,JE
暴露的青蛙骨骼肌的林格氏溶液制成高渗与甘油，然后返回的准备正常的林格氏液，结果在一个不可逆的解偶联的机械活动的肌肉。这种阻止肌肉收缩的技术常被用于研究在正常或高水平释放下的神经肌肉传递，而不受肌肉收缩的干扰。然而，很少有人知道甘油处理的过程中的发射机释放的影响。在本文中，我们报告了一项研究的结果，在这项研究中，我们研究了甘油治疗的影响，在重复刺激过程中，在青蛙缝匠肌神经肌肉接头的递质释放。我们发现，甘油治疗改变了刺激诱导的终板电位（EPP）振幅的变化，通常观察到在此突触重复刺激。这种效果，随着时间的推移逐渐增加，最多8小时后，去除甘油，可以解释突触前释放的递质量的变化。
Exposure of frog skeletal muscle to a Ringer solution made hyperosmotic with glycerol, followed by return of the preparation to a normal Ringer, results in an irreversible uncoupling of the mechanical activity of the muscle. This technique for preventing muscle contraction has often been used to study neuromuscular transmission under normal or high levels of release without inteference from muscle contraction. Little was known, however, about the effects of glycerol treatment on the process of transmitter release. In this paper we report the results of a study in which we examined the effects of glycerol treatment on transmitter release during repetitive stimulation at the frog sartorius neuromuscular junction. We found that glycerol treatment altered the stimulation-induced changes in end-plate potential (EPP) amplitude normally observed during repetitive stimulation at this synapse. This effect, which increased progressively with time for up to 8 h following removal of glycerol, could be accounted for by presynaptic changes in the amount of transmitter released.