Identification of programmed cell death in normal human skin tissues by using specific labelling of fragmented DNA
Identification of programmed cell death in normal human skin tissues by using specific labelling of fragmented DNA
复制标题
通过使用 DNA 片段的特异性标记来鉴定正常人体皮肤组织中的程序性细胞死亡
DOI：
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发表时间：
1994
影响因子：
10.3
通讯作者：
Y. Matsumoto
中科院分区：
文献类型：
--
作者：
Y. Tamada;H. Takama;T. Kitamura;K. Yokochi;Y. Nitta;T. Ikeya;Y. Matsumoto
用DNA片段原位特异性标记法研究了正常人皮肤组织中细胞程序性死亡（PCD）。这种标记方法在连续切片中清楚地染色了生长期毛囊球中的Henle层细胞核，并且在毛囊上部的内根鞘角质层细胞和Huxley层细胞的细胞核显示阳性染色。这种染色模式与三层中角化的顺序一致。位于皮脂腺中心的分化细胞的细胞核和颗粒角质形成细胞层的细胞核也被染色。这些结果表明，PCD可能在表皮和表皮附属物的终末分化中起关键作用。
Programmed cell death (PCD) in normal human skin tissues was studied by using in situ specific labelling of fragmented DNA. This labelling method clearly stained the nuclei of Henle's layer in the bulb of the anagen hair follicle in serial sections, and the nuclei of the inner root sheath cuticle cells and Huxley's layer cells showed positive staining in the upper part of the hair follicles. This staining pattern was consistent with the sequence of keratinization in the three layers. The nuclei of differentiated cells located at the centre of the sebaceous glands, and those of the granular keratinocyte layer, were also stained. These findings suggest that PCD might play a key role in the terminal differentiation of the epidermis and epidermal appendages.
影响因子：
6.5
作者：
MCCALL, CA;COHEN, JJ
通讯作者：
COHEN, JJ