Effects of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryo in vitro.
Effects of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryo in vitro.
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DOI：
10.1530/jrf.0.0960219
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发表时间：
1992-09
期刊：
影响因子：
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通讯作者：
Mohammad Hossein Nasr-Esfahani;N. Winston;Martin H. Johnson
中科院分区：
文献类型：
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作者：
Mohammad Hossein Nasr-Esfahani;N. Winston;Martin H. Johnson
从单细胞期到早期四细胞期的体外发育的前48小时的分析表明：(I)乙二胺四乙酸(EDTA)对F1和MF1胚胎的囊胚期培养具有主要的有利作用，(Ii)谷氨酰胺辅助MF1胚胎的发育到囊胚期，但对F1胚胎到囊胚期的发育没有帮助，并且可能是对线粒体氧化损伤的代谢反应的一部分，以及(Iii)在早期卵裂的某个时间暴露于葡萄糖对于囊胚的充分发育是必要的。所考察的培养条件对体外培养晚期2-细胞胚胎中活性氧浓度的增加没有显著影响，尽管体外培养的F1胚胎的过氧化氢浓度往往低于MF1胚胎。氧分压从20%下降到50%对培养到囊胚期或产生活性氧没有一致的影响。氨氧乙酸酯是转氨酶活性的抑制剂，可以阻止非阻塞型胚胎在第二个细胞周期的G2之后发育。因此，过渡金属的络合作用是克服体外发育障碍的最有效方法。
Analysis over the first 48 h of development in vitro from the one-cell stage to the early four-cell stage indicated that (i) ethylenediaminetetraacetic acid (EDTA) exerts the major beneficial effect on culture to the blastocyst stage of F1 and MF1 embryos, (ii) glutamine assists development of MF1, but not F1, embryos to the blastocyst stage and probably functions as part of a metabolic response to oxidative damage to mitochondria and (iii) exposure to glucose at some time during early cleavage is essential for full development to blastocysts. None of the culture conditions examined affected significantly the increase in concentration of reactive oxygen species in late two-cell embryos in vitro, although F1 embryos in vitro often had lower peroxide concentrations than MF1 embryos. A decline in oxygen tension from 20 to 50% had no consistent effect on culture to the blastocyst stage or production of reactive oxygen species. Aminooxyacetate, an inhibitor of transaminase activity, prevented non-blocking embryos from developing beyond G2 of the second cell cycle. It is concluded that the chelation of transitional metals provides the most effective method of overcoming the block to development in vitro.