Transcriptomic and proteomic analyses of pericycle cells of the maize primary root
Transcriptomic and proteomic analyses of pericycle cells of the maize primary root
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DOI：
10.1104/pp.107.106203
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发表时间：
2007-11-01
期刊：
影响因子：
7.4
通讯作者：
Hochholdinger, Frank
中科院分区：
文献类型：
--
作者：
Dembinsky, Diana;Woll, Katrin;Hochholdinger, Frank
每种植物细胞类型在不同的分化阶段表达独特的转录组和蛋白质组，这取决于其发育命运。本研究比较了玉米（Zea mays）第一次分裂和侧根发生前具有细胞周期能力的主根周膜细胞的基因表达和蛋白质积累。它们是唯一在离开分生区后仍保持分裂能力的根细胞。通过激光捕获显微切割分离近交系B73的周膜细胞。微阵列实验确定了32个基因优先表达的周细胞与所有其他的根细胞，已经离开顶端分生组织;选择性消减杂交确定了7个基因优先表达的周细胞与中央圆柱细胞的同一根区域。转录和蛋白质合成代表了这些周膜特异性基因中最丰富的功能类别。此外，701表达序列标签（EST）产生的周细胞和中央圆柱体细胞。其中，与蛋白质合成和细胞命运相关的转录本在周细胞与非周细胞中显著富集。此外，77个EST簇没有以前确定在玉米EST或基因组数据库进行了鉴定。最后，在通过二维电泳分离的最丰富的可溶性周膜蛋白中，通过电喷雾电离-串联质谱鉴定了20种蛋白质，从而定义了玉米周膜蛋白质组的参考数据集。其中，两种蛋白质优先表达于周膜。总之，这些周鞘特异性基因表达实验定义了在细胞周期能力的周鞘细胞在其第一次分裂之前的特化过程中的不同分子事件，并证明周鞘特化和侧根起始可能由不同的基因组控制。
Each plant cell type expresses a unique transcriptome and proteome at different stages of differentiation dependent on its developmental fate. This study compared gene expression and protein accumulation in cell- cycle- competent primary root pericycle cells of maize ( Zea mays) prior to their first division and lateral root initiation. These are the only root cells that maintain the competence to divide after they leave the meristematic zone. Pericycle cells of the inbred line B73 were isolated via laser capture microdissection. Microarray experiments identified 32 genes preferentially expressed in pericycle versus all other root cells that have left the apical meristem; selective subtractive hybridization identified seven genes preferentially expressed in pericycle versus central cylinder cells of the same root region. Transcription and protein synthesis represented the most abundant functional categories among these pericycle- specific genes. Moreover, 701 expressed sequence tags ( ESTs) were generated from pericycle and central cylinder cells. Among those, transcripts related to protein synthesis and cell fate were significantly enriched in pericycle versus nonpericycle cells. In addition, 77 ESTclusters not previously identified in maize ESTs or genomic databases were identified. Finally, among the most abundant soluble pericycle proteins separated via two-dimensional electrophoresis, 20 proteins were identified via electrospray ionization- tandem mass spectrometry, thus defining a reference dataset of the maize pericycle proteome. Among those, two proteins were preferentially expressed in the pericycle. In summary, these pericycle- specific gene expression experiments define the distinct molecular events during the specification of cell- cycle- competent pericycle cells prior to their first division and demonstrate that pericycle specification and lateral root initiation might be controlled by a different set of genes.