A fully automated flow-based approach for accelerated peptide synthesis
A fully automated flow-based approach for accelerated peptide synthesis
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DOI：
10.1038/nchembio.2318
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发表时间：
2017-05-01
影响因子：
14.8
通讯作者：
Pentelute, Bradley L.
中科院分区：
文献类型：
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作者：
Mijalis, Alexander J.;Thoma, Dale A., III;Pentelute, Bradley L.
在这里，我们报告了一个完全自动化的，基于流动的固相多肽合成方法，酰胺键形成在7秒内，每个氨基酸残基的总合成时间为40秒。粗肽纯度和分离的产率与标准批次固相肽合成的那些相当。在满负荷运行时，这种方法每年可以产生数万个单独的30聚体肽。
Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year.