Template boundary in a yeast telomerase specified by RNA structure.
Template boundary in a yeast telomerase specified by RNA structure.
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DOI：
10.1126/science.288.5467.863
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发表时间：
2000-05
期刊：
影响因子：
56.9
通讯作者：
Y. Tzfati;Tracy B. Fulton;J. Roy;Elizabeth H. Blackburn
中科院分区：
文献类型：
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作者：
Y. Tzfati;Tracy B. Fulton;J. Roy;Elizabeth H. Blackburn
端粒酶核糖核蛋白有一个遗传学上不同的RNA亚基，其中含有一个短的模板端粒DNA合成。为了了解端粒酶RNA如何参与端粒合成的机制方面，我们研究了模板附近的保守二级结构。这种结构的破坏导致DNA合成超出正常的模板边界，导致端粒序列改变，端粒缩短和细胞生长缺陷。补偿性突变恢复了正常的端粒酶功能。因此，RNA的结构，而不是它的序列，指定了模板的边界。这项研究揭示了一种RNA结构在端粒酶的酶促作用中的特殊功能。
The telomerase ribonucleoprotein has a phylogenetically divergent RNA subunit, which contains a short template for telomeric DNA synthesis. To understand how telomerase RNA participates in mechanistic aspects of telomere synthesis, we studied a conserved secondary structure adjacent to the template. Disruption of this structure caused DNA synthesis to proceed beyond the normal template boundary, resulting in altered telomere sequences, telomere shortening, and cellular growth defects. Compensatory mutations restored normal telomerase function. Thus, the RNA structure, rather than its sequence, specifies the template boundary. This study reveals a specific function for an RNA structure in the enzymatic action of telomerase.