Spatial regulation of SpMTA metallothionein gene expression in sea urchin embryos by a regulatory cassette in intron 1.
Spatial regulation of SpMTA metallothionein gene expression in sea urchin embryos by a regulatory cassette in intron 1.
复制标题
海胆胚胎中 SpMTA 金属硫蛋白基因表达的空间调控通过内含子 1 中的调控盒。
DOI：
10.1016/0925-4773(94)00330-p
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发表时间：
1995
影响因子：
2.6
通讯作者：
Parker,HR
中科院分区：
文献类型：
--
作者：
Nemer,M;Stuebing,EW;Bai,G;Parker,HR
海胆Strongylocentrotus purpuratus的SpMTA金属硫蛋白（MT）基因的表达限制在原肠胚和pluteus幼虫的离口外胚层。SpMTA基因5′侧翼区近端1.6kb的长度和第一内含子1.12kb的长度足以使其在转基因胚胎中正确表达。这种有限的空间表达在很大程度上消除了内部405-bp区域的内含子删除。在该区域内是295-bp的基因组重复的转座子样区段（Nemer等人，1993），其含有几个与其它基因启动子中所设调控元件高度同源的序列基序（Thiebaud等，1990年）。在这个明显的调控盒中的P3 A和P5位点通过竞争以相对高的亲和力结合相同的核因子，通过它们在CyIIIa肌动蛋白启动子中的对应位点结合。
The SpMTA metallothionein (MT) gene of the sea urchin Strongylocentrotus purpuratus is restricted in its expression to the aboral ectoderm in gastrulae and pluteus larvae. The proximal 1.6 kb of the 5′-flanking region together with the 1.12-kb first intron of the SpMTA gene are sufficient for its correct cell-type specific expression in transgenic embryos. This restricted spatial expression is largely eliminated by deletion of an interior 405-bp region in the intron. Within this region is a 295-bp, genomically repetitive, transposon-like segment (Nemer et al., 1993), containing several sequence motifs highly homologous to posited regulatory elements in the promoters of other genes (Thiebaud et al., 1990). The P3A and P5 sites in this apparent regulatory cassette were shown through competition to bind with relatively high affinities the same nuclear factors, bound by their counterpart sites in the CyIIIa actin promoter.