BREFELDIN-A AFFECTS EARLY EVENTS BUT DOES NOT AFFECT LATE EVENTS ALONG THE EXOCYTIC PATHWAY IN PANCREATIC ACINAR-CELLS
BREFELDIN-A AFFECTS EARLY EVENTS BUT DOES NOT AFFECT LATE EVENTS ALONG THE EXOCYTIC PATHWAY IN PANCREATIC ACINAR-CELLS
复制标题
DOI：
10.1073/pnas.89.15.7242
复制
发表时间：
1992-08-01
影响因子：
11.1
通讯作者：
FARQUHAR, MG
中科院分区：
文献类型：
--
作者：
HENDRICKS, LC;MCCLANAHAN, SL;FARQUHAR, MG
布雷菲德菌素A（Brefeldin A，BFA）阻断蛋白质从内质网（内质网，内质网）输出到高尔基复合体，并且在一些培养细胞中引起高尔基复合体的解体，同时使驻留的高尔基蛋白重新定位到内质网。尚不清楚分泌过程中的后续步骤是否受到影响。我们以前已经表明，在BFA处理的大鼠胰腺小叶，有没有可检测到的高尔基体蛋白的ER的搬迁，虽然高尔基体池被迅速拆除，小的光滑囊泡组成的真正的高尔基体残余物和相关的囊泡载体的集群持续存在，即使与长期的BFA曝光。我们现在报告了BFA通过胰腺外分泌细胞分泌途径对蛋白质转运的影响;我们用[S-35]蛋氨酸脉冲标记胰腺小叶，然后在加入BFA之前追踪不同时间。当在脉冲时加入BFA时，处理的小叶释放
Brefeldin A (BFA) blocks protein export from the endoplasmic reticulum (ER) to Golgi complex and causes dismantling of the Golgi complex with relocation of resident Golgi proteins to the ER in some cultured cells. It is not known whether later steps in the secretory process are affected. We previously have shown that in BFA-treated rat pancreatic lobules, there is no detectable relocation of Golgi proteins to the ER and, although Golgi cisternae are rapidly dismantled, clusters of small smooth vesicles consisting of both bona fide Golgi remnants and associated vesicular carriers persist even with prolonged BFA exposure. We now report the effects of BFA on transport of proteins through the secretory pathway in exocrine pancreatic cells; we pulse-labeled pancreatic lobules with [S-35]methionine and then chased for various times before adding BFA. When BFA was added at pulse, treated lobules released