Developmental stage-specific expression and tissue distribution of pierisin-1, a guanine-specific ADP-ribosylating toxin, in Pieris rapae
Developmental stage-specific expression and tissue distribution of pierisin-1, a guanine-specific ADP-ribosylating toxin, in Pieris rapae
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DOI：
10.1016/j.cbpb.2004.07.010
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发表时间：
2004-10-01
影响因子：
2.3
通讯作者：
Wakabayashi, K
中科院分区：
文献类型：
--
作者：
Watanabe, M;Nakano, T;Wakabayashi, K
菜粉蝶(Pieris Rapae)产生一种针对哺乳动物细胞的凋亡诱导蛋白Pierisin-1。为了阐明Pierisin-1在菜青虫中的生物学作用，对其在不同发育阶段的表达进行了研究。在1龄幼虫中检测到较低水平的Pierisin-1基因和蛋白。在生长至5龄幼虫阶段，mRNA的量稳步增加，达到初始水平的50-100倍。化蛹前迅速下降。在蛹和成虫中的mRNA表达水平与一龄幼虫一样低。从1龄到5龄幼虫，PIERISIN-1蛋白的表达水平也增加了约100倍，然后在蛹期逐渐下降，降幅超过90%。Pierisin-1免疫组织化学染色显示，该蛋白主要定位于五龄幼虫和早期蛹的脂肪体。虽然染色强度较低，但幼虫和成虫早期龄期的脂肪体也呈阳性。此外，对分离的脂肪体和其他组织样本的检查与上述观察结果一致。因此，结果表明，在幼虫的后期，Pierisin-1的mRNA高度表达，并且该蛋白在化蛹期间持续存在的脂肪体中积累。(C)2004 Elsevier Inc.保留所有权利。
The cabbage butterfly (Pieris rapae) produces pierisin-1, an apoptosis-inducing protein against mammalian cells. In order to clarify the biological role of pierisin-1 in P. rapae, its expression during developmental stages was examined. Low levels of pierisin-1 mRNA and protein were detected in first-instar larvae. During growth until the fifth-instar larval stage, the amounts of the mRNA steadily increased to reach about 50-100 times the initial level. Then it rapidly decreased before pupation. The levels of mRNA in the pupae and the adults were as low as in the first-instar larvae. Levels of pierisin-1 protein also increased around 100 times from the first-instar to the fifth-instar larvae and then gradually decreased by over 90% during the pupal stage. Immunostaining of pierisin-1 demonstrated the protein to be mainly located in fat bodies of fifth-instar larvae and early-phase pupae. Although the staining intensity was low, fat bodies of early instars of the larvae and adults were also found to be positive. Moreover, examination of isolated fat body and other tissue samples of the insects were consistent with the above observations. Thus, the results indicate that mRNA of pierisin-1 was highly expressed in late stages of larvae, and that the protein accumulated in fat bodies where it persists during pupation. (C) 2004 Elsevier Inc. All rights reserved.