STRUCTURE AND EXPRESSION OF 2 PLANT GENES ENCODING CHROMOPLAST-SPECIFIC PROTEINS - OCCURRENCE OF PARTIALLY SPLICED TRANSCRIPTS
STRUCTURE AND EXPRESSION OF 2 PLANT GENES ENCODING CHROMOPLAST-SPECIFIC PROTEINS - OCCURRENCE OF PARTIALLY SPLICED TRANSCRIPTS
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DOI：
10.1006/bbrc.1994.1350
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发表时间：
1994-03-30
影响因子：
3.1
通讯作者：
KUNTZ, M
中科院分区：
文献类型：
--
作者：
DERUERE, J;BOUVIER, F;KUNTZ, M
辣椒素和辣椒红素-辣椒红素合成酶基因在辣椒果实成熟过程中特异表达，导致这两种蛋白质在色素体中积累。在这里，我们报告的第一次克隆的基因组DNA片段编码这两种酶，以及含有上游区域的DNA片段，这是潜在的参与这些基因的表达调控。虽然辣椒红素-辣椒红素合酶基因是不间断的，但辣椒素基因被两个内含子中断，第一个内含子被无效剪接。未剪接的转录本的出现显然与控制Bisocin或替代多肽合成的转录后机制无关。本工作为植物基因激活和内含子剪接的研究提供了工具。 (C)1994年出版社出版。
The genes encoding fibrillin and capsanthin-capsorubin synthase are specifically expressed during fruit ripening in Capsicum annuum, leading to the accumulation of these two proteins in chromoplasts. Here, we report for the first time the cloning of genomic DNA fragments encoding these two enzymes, as well as DNA fragments containing upstream regions which are potentially involved in the regulation of the expression of these genes. While the capsanthin-capsorubin synthase gene is uninterrupted, the fibrillin gene is interrupted by two introns, the first one being inefficiently spliced. Occurrence of unspliced transcripts is apparently not related to a post-transcriptional mechanism controlling the synthesis of fibrillin or an alternative polypeptide. This work provides tools for studies on gene activation and intron splicing in plants. (C) 1994 Academic Press, Inc.