NON-REACTIVITY OF THE SELENOENZYME GLUTATHIONE-PEROXIDASE WITH ENZYMATICALLY HYDROPEROXIDIZED PHOSPHOLIPIDS
NON-REACTIVITY OF THE SELENOENZYME GLUTATHIONE-PEROXIDASE WITH ENZYMATICALLY HYDROPEROXIDIZED PHOSPHOLIPIDS
复制标题
DOI：
10.1111/j.1432-1033.1983.tb07687.x
复制
发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
WENDEL, A
中科院分区：
文献类型：
--
作者：
GROSSMANN, A;WENDEL, A
从牛红细胞中将含硒谷胱甘肽过氧化物酶（EC 1.11.1.9）纯化6000倍至表观均一性。脂肪氧合酶（EC 1.13.11.12）从大豆丙酮粉中富集20倍。用脂氧合酶将亚油酸过氧化，然后用作谷胱甘肽过氧化物酶反应中的底物。用合成的1，2-二亚油酰基-L-α-甘油磷酸胆碱和天然牛心心磷脂。仅在[猪胰腺]磷脂酶A2（EC 3.1.1.4）酶促攻击后，过氧化磷脂才与谷胱甘肽过氧化物酶反应。这一结果意味着，谷胱甘肽过氧化物酶的膜保护功能，包括前磷脂酶的行动，并排除了这种酶与膜结合的脂质氢过氧化物的直接相互作用。
Se-containing glutathione peroxidase (EC 1.11.1.9) was purified 6000-fold from bovine red blood cells to apparent homogeneity. Lipoxygenase (EC 1.13.11.12) was enriched 20-fold from soybean acetone powder. Linoleic acid was peroxidized with lipoxygenase and then used as a substrate in the glutathione peroxidase reaction. Analogous experiments were conducted with synthetic 1,2-dilinoleoyl-L-.alpha.-glycerophosphocholine and with natural bovine heart cardiolipin. The peroxidized phospholipids were reactive with glutathione peroxidase only after enzymatic attack by [pig pancreas] phospholipase A2 (EC 3.1.1.4). This result implies that the membrane-protective function of glutathione peroxidase includes preceding phospholipase action and excludes a direct interaction of this enzyme with membrane-bound lipid hydroperoxides.