SYNTHESIS AND TRANSPORT OF STORAGE PROTEINS BY TESTES IN HELIOTHIS-VIRESCENS
SYNTHESIS AND TRANSPORT OF STORAGE PROTEINS BY TESTES IN HELIOTHIS-VIRESCENS
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DOI：
10.1002/arch.940140304
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发表时间：
1990-01-01
影响因子：
2.2
通讯作者：
MALONE, C
中科院分区：
文献类型：
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作者：
MILLER, SG;LECLERC, RF;MALONE, C
研究了绿色日光夜蛾睾丸鞘合成76,000-MR和82,000-MR两个贮藏蛋白亚基的过程。与脂肪体一样，睾丸鞘细胞也分泌糖化的储存蛋白，组装成六聚体。脂肪体是大睾丸外脂肪合成的主要场所。睾丸鞘在几个重要方面与脂肪体不同，包括不能合成丰富的(血淋巴中)74,000-MR储存蛋白，其76,000-MR多肽的表达相对减少，以及在幼虫发育过程中没有从睾丸管腔吸收储存蛋白。囊泡细胞还通过吞噬睾丸液主动输入82,000-MR储存蛋白，并将其转移到发育中的精子细胞。与其他类型的细胞以细胞质颗粒的形式隔离储存蛋白质不同，它们在精子细胞中的定位完全是线粒体。这些观察表明，储存蛋白基因的表达受到组织特异性的调控，并揭示了它们在发育过程中的运输以及可能的利用和功能的新途径。
The synthesis of two storage protein subunits, 76,000-Mr and 82,000-Mr polypeptides, by the testes sheath has been studied in Heliothis virescens. Like fat body, which is the primary site of synthesis for the large extratesticular pool, cells of the testes sheath secrete glycosylated storage proteins assembled into hexamers. The testis sheath differed from fat body in several important respects, including the failure to synthesize an abundant (in the hemolymph) 74,000-Mr storage protein, its relatively reduced expression of the 76,000-Mr polypeptide, and the absence of resorption of storage proteins from the lumen of the testis during pupal development. Cyst cells were also shown to import actively the 82,000-Mr storage protein by pinocytosis of testicular fluid and transfer it to the developing spermatids. Unlike other cell types that sequester storage proteins in the form of cytoplasmic granules, their localization within spermatids was exclusively mitochondrial. These observations suggest that expression of the storage protein genes is regulated tissue specifically and reveal novel pathways from their transport and, perhaps, utilization and function during development.