P-element invasion fuels molecular adaptation in laboratory populations of Drosophila melanogaster.
P-element invasion fuels molecular adaptation in laboratory populations of Drosophila melanogaster.
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P 元素入侵促进了实验室黑腹果蝇种群的分子适应。
DOI：
10.1093/evolut/qpad017
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kelleher,Erin
中科院分区：
文献类型：
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作者：
Wang,Luyang;Zhang,Shuo;Hadjipanteli,Savana;Saiz,Lorissa;Nguyen,Lisa;Silva,Efren;Kelleher,Erin
转座元件(TES)是一种可移动的遗传寄生虫，经常通过水平转移入侵新的寄主基因组。入侵的TES通常表现出一系列的转位，随后随着寄主内抑制的发展，转位速率降低。我们重建了水平转移OFP元件DNA转座子到果蝇黑腹宿主中，并观察了在不同温度下饲养的复制实验室种群中TE拷贝的扩张和宿主抑制的进化。我们观察到，当维持在高温下的种群在TE入侵后迅速灭绝时，那些保持在较低温度下的种群仍然存在，允许TE传播和宿主抑制的进化。我们还令人惊讶地发现，入侵群体经历了OFP元件反复插入特定的长非编码RNA，lncRNA：CR43651，并且这些插入等位基因在实验群体中以异常高的频率分离，表明正选择。我们认为，除了驱动抑制的进化外，入侵TES的转位爆发还可以驱动分子适应。
Transposable elements (TEs) are mobile genetic parasites that frequently invade new host genomes through horizontal transfer. Invading TEs often exhibit a burst of transposition, followed by reduced transposition rates as repression evolves in the host. We recreated the horizontal transfer ofP-element DNA transposons into aDrosophila melanogasterhost and followed the expansion of TE copies and evolution of host repression in replicate laboratory populations reared at different temperatures. We observed that while populations maintained at high temperatures rapidly go extinct after TE invasion, those maintained at lower temperatures persist, allowing for TE spread and the evolution of host repression. We also surprisingly discovered that invaded populations experienced recurrent insertion ofP-elements into a specific long non-coding RNA,lncRNA:CR43651,and that these insertion alleles are segregating at unusually high frequency in experimental populations, indicative of positive selection. We propose that, in addition to driving the evolution of repression, transpositional bursts of invading TEs can drive molecular adaptation.