The genome of the Erwinia amylovora phage PhiEaH1 reveals greater diversity and broadens the applicability of phages for the treatment of fire blight
The genome of the Erwinia amylovora phage PhiEaH1 reveals greater diversity and broadens the applicability of phages for the treatment of fire blight
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DOI：
10.1111/1574-6968.12319
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发表时间：
2014-01-01
影响因子：
2.1
通讯作者：
Kovacs, Tamas
中科院分区：
文献类型：
--
作者：
Meczker, Katalin;Doemoetoer, Dora;Kovacs, Tamas
梨火疫病菌是火疫病的致病菌。这项研究对噬菌体 PhiEaH1 的完整基因组进行了分析，该噬菌体是从匈牙利一棵受梨火霉感染的苹果树周围的土壤中分离出来的。其基因组大小为218kb，包含244个ORF。 PhiEaH1 是来自 Siphoviridae 家族的第二个感染淀粉样肠球菌的噬菌体，其完整基因组序列已确定。除了 PhiEaH2 之外，PhiEaH1 是 Erwiphage 的另一个活性成分，Erwiphage 是市场上第一种针对梨火疫霉的噬菌体杀虫剂。本研究中的比较基因组分析表明，PhiEaH1 不仅与先前测序的 10 种属于其他噬菌体家族的梨火疫病菌噬菌体不同，而且与 PhiEaH2 也不同。对更多管病毒科噬菌体基因组进行测序可能会揭示进一步的多样性，为开发更有效的生物防治剂、针对商业水果作物欧文氏菌火疫病的噬菌体混合物提供机会。
The enterobacterium Erwinia amylovora is the causal agent of fire blight. This study presents the analysis of the complete genome of phage PhiEaH1, isolated from the soil surrounding an E.amylovora-infected apple tree in Hungary. Its genome is 218kb in size, containing 244 ORFs. PhiEaH1 is the second E.amylovora infecting phage from the Siphoviridae family whose complete genome sequence was determined. Beside PhiEaH2, PhiEaH1 is the other active component of Erwiphage, the first bacteriophage-based pesticide on the market against E.amylovora. Comparative genome analysis in this study has revealed that PhiEaH1 not only differs from the 10 formerly sequenced E.amylovora bacteriophages belonging to other phage families, but also from PhiEaH2. Sequencing of more Siphoviridae phage genomes might reveal further diversity, providing opportunities for the development of even more effective biological control agents, phage cocktails against Erwinia fire blight disease of commercial fruit crops.