Polymorphism at GSTM1, GSTM3 and GSTT1 gene loci and susceptibility to oral cancer in an Indian population
Polymorphism at GSTM1, GSTM3 and GSTT1 gene loci and susceptibility to oral cancer in an Indian population
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DOI：
10.1093/carcin/23.5.803
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发表时间：
2002-05-01
期刊：
影响因子：
4.7
通讯作者：
Bhisey, RA
中科院分区：
文献类型：
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作者：
Buch, SC;Notani, PN;Bhisey, RA
本研究评估了习惯于使用无烟烟草、比迪烟或香烟的印度人中GSTM 1、GSTM 3和GSTT 1基因位点的遗传多态性对口腔癌风险的影响。应用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析方法，对297例恶性肿瘤患者和450例健康对照者的白色血细胞DNA进行了分析。采用Logistic回归分析，将终生烟草暴露评估为与GST基因位点多态性相关的危险因素。口腔癌患者和对照组之间GSTM 3和GSTT 1基因型分布无显着差异。相反，GSTM 1缺失基因型患者的风险显著增加3倍（年龄校正OR = 3.2，95%CI 2.4-4.3）。GSTM 1无效基因型对口腔癌风险的影响也在不同烟草习惯的个体中进行了分析。与GSTM 1无效基因型相关的优势比在烟草咀嚼者中为3.7（95%CI 2.0-7.1），在bidi吸烟者中为3.7（5%CI 1.3-7.9），在香烟吸烟者中为5.7（95%CI 2.0-16.3）。此外，增加咀嚼烟草的终生暴露似乎与GSTM 1缺失个体口腔癌风险增加2倍相关。结果表明，GSTM 1无效基因型是印度烟草习惯者口腔癌发展的危险因素。
This study evaluates the influence of genetic polymorphism at GSTM1, GSTM3 and GSTT1 gene loci on oral cancer risk among Indians habituated to the use of, smokeless tobacco, bidi or cigarette. DNA extracted from white blood cells of 297 cancer patients and 450 healthy controls by the proteinase K phenol-chloroform extraction procedure were analyzed by the polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) analyses. Lifetime tobacco exposure was evaluated as a risk factor in relation to the polymorphism at the GST gene loci using logistic regression analysis. There was no significant difference in the distribution of the GSTM3 and GSTT1 genotypes between oral cancer patients and controls. In contrast, a significant 3-fold increase in risk was seen for patients with the GSTM1 null genotype (age adjusted OR = 3.2, 95% CI 2.4-4.3). The impact of the GSTM1 null genotype on oral cancer risk was also analyzed in separate groups of individuals with different tobacco habits. The odds ratio associated with the GSTM1 null genotype was 3.7 (95% CI 2.0-7.1) in tobacco chewers, 3.7 (5% CI 1.3-7.9) in bidi smokers and 5.7 (95% CI 2.0-16.3) in cigarette smokers. Furthermore, increased lifetime exposure to chewing tobacco appeared to be associated with a 2-fold increase in oral cancer risk in GSTM1 null individuals. The results suggest that the GSTM1 null genotype is a risk factor for development of oral cancer among Indian tobacco habitues.