In vitro and in vivo degradation correlations for polyurethane foams with tunable degradation rates
In vitro and in vivo degradation correlations for polyurethane foams with tunable degradation rates
复制标题
DOI：
10.1002/jbm.a.37504
复制
发表时间：
2023-02-08
影响因子：
4.9
通讯作者：
Monroe，Mary Beth Browning
中科院分区：
文献类型：
--
作者：
Vakil，Anand Utpal;Petryk，Natalie Marie;Monroe，Mary Beth Browning
聚氨酯泡沫提供了一个可调的生物材料平台，具有在一系列再生医学应用中使用的潜力。实现支架降解速率和组织向内生长之间的平衡对于成功的伤口愈合至关重要，并且需要进行重要的体内测试来了解这些过程。强有力的体外试验可以最大限度地减少收集可靠数据所需的动物数量;然而，很难准确选择可以有效模拟体内结果的体外降解条件。为此，我们使用不同浓度的过氧化氢对具有可调降解速率的多孔形状记忆聚氨酯泡沫的降解进行了全面的体外评估，以确定密切模拟体内降解速率的介质。在体外1%、2%或3%过氧化氢中以及在体内Sprague道利大鼠皮下囊袋中研究了12周的材料降解。我们发现，最佳预测体内降解速率的体外降解条件根据聚合物降解机制的数量和聚合物亲水性而变化。也就是说，通过水解和氧化降解的亲水性材料需要较低浓度的过氧化氢（1%）来模拟体内降解速率，而仅通过氧化降解的疏水性支架需要较高浓度的过氧化氢（3%）来模拟体内降解。该信息可用于合理选择体外降解条件，以便在动物模型中进行表征之前准确鉴定体内降解速率。
Polyurethane foams present a tunable biomaterial platform with potential for use in a range of regenerative medicine applications. Achieving a balance between scaffold degradation rates and tissue ingrowth is vital for successful wound healing, and significant in vivo testing is required to understand these processes. Vigorous in vitro testing can minimize the number of animals that are required to gather reliable data; however, it is difficult to accurately select in vitro degradation conditions that can effectively mimic in vivo results. To that end, we performed a comprehensive in vitro assessment of the degradation of porous shape memory polyurethane foams with tunable degradation rates using varying concentrations of hydrogen peroxide to identify the medium that closely mimics measured in vivo degradation rates. Material degradation was studied over 12 weeks in vitro in 1%, 2%, or 3% hydrogen peroxide and in vivo in subcutaneous pockets in Sprague Dawley rats. We found that the in vitro degradation conditions that best predicted in vivo degradation rates varied based on the number of mechanisms by which the polymer degraded and the polymer hydrophilicity. Namely, more hydrophilic materials that degrade by both hydrolysis and oxidation require lower concentrations of hydrogen peroxide (1%) to mimic in vivo rates, while more hydrophobic scaffolds that degrade by oxidation alone require higher concentrations of hydrogen peroxide (3%) to model in vivo degradation. This information can be used to rationally select in vitro degradation conditions that accurately identify in vivo degradation rates prior to characterization in an animal model.