Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images?
Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images?
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DOI：
10.1104/pp.16.00454
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发表时间：
2016-06-01
期刊：
影响因子：
7.4
通讯作者：
Ross, John J.
中科院分区：
文献类型：
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作者：
Cook, Sam D.;Nichols, David S.;Ross, John J.
植物中主要生长素（吲哚-3-乙酸[IAA]）的生物合成最近已被阐明，并且被认为涉及Trp到吲哚-3-乙酸到IAA的顺序转化。然而，导致生长素苯乙酸（PAA）研究较少的途径仍然不清楚。在这里，我们提出的证据，从代谢实验表明，PAA是合成的氨基酸苯丙氨酸，通过苯丙酮酸。在豌豆（豌豆），逆反应，苯丙酮酸苯丙氨酸，也被证明。然而，尽管导致IAA和PAA的途径之间的相似性，豌豆和玉米（玉米）突变体的证据表明，IAA生物合成酶不是PAA生物合成的主要酶。相反，我们确定了一个假定的芳香族氨基转移酶（PsArAT）豌豆，可能在PAA合成途径的功能。
The biosynthesis of the main auxin in plants (indole-3-acetic acid [IAA]) has been elucidated recently and is thought to involve the sequential conversion of Trp to indole-3-pyruvic acid to IAA. However, the pathway leading to a less well studied auxin, phenylacetic acid (PAA), remains unclear. Here, we present evidence from metabolism experiments that PAA is synthesized from the amino acid Phe, via phenylpyruvate. In pea (Pisum sativum), the reverse reaction, phenylpyruvate to Phe, is also demonstrated. However, despite similarities between the pathways leading to IAA and PAA, evidence from mutants in pea and maize (Zea mays) indicate that IAA biosynthetic enzymes are not the main enzymes for PAA biosynthesis. Instead, we identified a putative aromatic aminotransferase (PsArAT) from pea that may function in the PAA synthesis pathway.