Inhibition of Streptococcus mutans by the antibiotic streptozotocin: mechanisms of uptake and the selection of carbohydrate-negative mutants.
Inhibition of Streptococcus mutans by the antibiotic streptozotocin: mechanisms of uptake and the selection of carbohydrate-negative mutants.
复制标题
抗生素链脲佐菌素对变形链球菌的抑制：摄取机制和碳水化合物阴性突变体的选择。
DOI：
10.1128/iai.58.2.543-549.1990
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Lengeler,JW
中科院分区：
文献类型：
--
作者：
Jacobson,GR;Poy,F;Lengeler,JW
抗生素链脲霉素[2-脱氧-2-（3-甲基-3-亚硝基脲基）-D-吡喃葡萄糖苷]是N-乙酰葡糖胺（NAG）的类似物，已被证明可用于选择碳水化合物阴性和营养缺陷型细菌突变体。我们已经调整了这种方法用于口腔病原体变形链球菌，革兰氏阳性，耐氧厌氧菌，主要使用碳水化合物作为碳源的增长。链脲佐菌素选择性地杀死生长中的S.变异株GS-5，在适当的条件下，它可以减少活跃生长的培养物中的活细胞数，减少因子为10（3）至10（4）。然而，与肠道细菌不同的是，链脲佐菌素似乎被S.通过NAG特异性系统和也参与葡萄糖、果糖和甘露糖摄取的相对非特异性系统两者，对变形杆菌产生抑制作用。结合链脲佐菌素选择和筛选程序，包括含有三苯基四唑氯化物的指示板，我们开发了一个通用的方法分离的碳水化合物阴性和营养缺陷型突变体的S。变种人通过使用这个程序分离的多效性和特定的碳水化合物阴性突变体的初步表征。
The antibiotic streptozotocin [2-deoxy-2-(3-methyl-3-nitrosoureido)-D-glucopyranoside], an analog of N-acetylglucosamine (NAG), has been shown to be useful for the selection of carbohydrate-negative and auxotrophic bacterial mutants. We have adapted this method for use with the oral pathogen Streptococcus mutans, a gram-positive, aerotolerant anaerobe that uses predominantly carbohydrates as carbon sources for growth. Streptozotocin selectively kills growing cells of S. mutans GS-5, and under appropriate conditions it can reduce the number of viable cells in actively growing cultures by a factor of 10(3) to 10(4). However, unlike in enteric bacteria, which take up this antibiotic by a single NAG-specific transport system, streptozotocin appears to be taken up in S. mutans by both a NAG-specific system and a relatively nonspecific system that is also involved in glucose, fructose, and mannose uptake. Combining streptozotocin selection and a screening procedure involving indicator plates containing triphenyl-tetrazolium chloride, we developed a general method for the isolation of carbohydrate-negative and auxotrophic mutants of S. mutans. A preliminary characterization of both pleiotropic and specific carbohydrate-negative mutants isolated by using this procedure is presented.