Micropatterning fluid lipid bilayers on solid supports
Micropatterning fluid lipid bilayers on solid supports
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DOI：
10.1126/science.275.5300.651
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发表时间：
1997-01-31
期刊：
影响因子：
56.9
通讯作者：
Boxer, SG
中科院分区：
文献类型：
--
作者：
Groves, JT;Ulman, N;Boxer, SG
光刻图案化的网格的光致抗蚀剂，氧化铝，或氧化硅基板上的金被用来分区支持的脂质双层成微米级阵列的隔离流体膜corpus。观察到荧光标记的脂质在每个膜围栏内自由扩散，但受到微图案化屏障的限制。通过选择性光漂白来改变个体皮质中荧光探针分子的浓度，以产生具有不同组成的流体膜贴片阵列。施加平行于表面的电场，在皮质中诱导带电膜组分的稳态浓度梯度，除了产生具有连续变化的组成的膜的补丁之外，这些梯度提供了获取关于分子性质的信息的本质上平行的手段，例如在个体皮质中的扩散系数。
Lithographically patterned grids of photoresist, aluminum oxide, or gold on oxidized silicon substrates were used to partition supported lipid bilayers into micrometer-scale arrays of isolated fluid membrane corrals. Fluorescently labeled lipids were observed to diffuse freely within each membrane corral but were confined by the micropatterned barriers. The concentrations of fluorescent probe molecules in individual corrals were altered by selective photobleaching to create arrays of fluid membrane patches with differing compositions. Application of an electric field parallel to the surface induced steady-state concentration gradients of charged membrane components in the corrals, In addition to producing patches of membrane with continuously varying composition, these gradients provide an intrinsically parallel means of acquiring information about molecular properties such as the diffusion coefficient in individual corrals.