Divergence population genetic analysis of hybridization between rhesus and cynomolgus macaques
Divergence population genetic analysis of hybridization between rhesus and cynomolgus macaques
复制标题
DOI：
10.1111/j.1365-294x.2009.04212.x
复制
发表时间：
2009-06-01
影响因子：
4.9
通讯作者：
Kohn, Michael H.
中科院分区：
文献类型：
--
作者：
Stevison, Laurie S.;Kohn, Michael H.
恒河猴（Macaca mulatta）和食蟹猴（M. fascicularis）猕猴毗邻印度支那，在那里他们似乎杂交。我们使用已发表的和新产生的DNA序列，从19个位点跨越类似的20 kb，以测试这些猕猴物种之间是否发生了基因渗入。我们研究了基因渗入在核DNA的水平和祖先的多态性或种间基因流的不完全谱系排序之间的区别。我们通过将我们的数据拟合到在软件IMa中实现的隔离模型来实现发散群体遗传学方法。假设没有基因从恒河猴流入食蟹猴的模型被拒绝（P = 1.99 x 10（-8））。在这个方向上的基因流估计为2Nm，与1.2相似，而在相反方向上的基因流与零无显著差异（P = 0.16）。物种之间的分歧时间估计为130万年。平衡选择，一个特殊的情况下，不完整的排序，被考虑在内，以及潜在的杂交圈养。参数估计值在数据子集的分析之间存在差异，尽管我们仍然拒绝了隔离模型。地理抽样的数据，其中来自印度支那的食蟹猴样本被排除在外，揭示了丢失的基因流的签名，表明种间基因流仅限于大陆印度支那。我们的研究结果与其他人的研究结果相结合，证明了未来对这两种基因组启动灵长类动物生殖障碍和网状进化遗传学的详细分析是合理的。恒河猴和食蟹猴自然杂交的未来研究将扩大灵长类物种形成研究的系统库。
The geographic ranges of rhesus (Macaca mulatta) and cynomolgus (M. fascicularis) macaques adjoin in Indochina where they appear to hybridize. We used published and newly generated DNA sequences from 19 loci spanning similar to 20 kb to test whether introgression has occurred between these macaque species. We studied introgression at the level of nuclear DNA and distinguished between incomplete lineage sorting of ancestral polymorphisms or interspecific gene flow. We implemented a divergence population genetics approach by fitting our data to an isolation model implemented in the software IMa. The model that posits no gene flow from the rhesus into the cynomolgus macaque was rejected (P = 1.99 x 10(-8)). Gene flow in this direction was estimated as 2Nm similar to 1.2, while gene flow in the reverse direction was nonsignificantly different from zero (P = 0.16). The divergence time between species was estimated as similar to 1.3 million years. Balancing selection, a special case of incomplete sorting, was taken into consideration, as well as potential crossbreeding in captivity. Parameter estimates varied between analyses of subsets of data, although we still rejected isolation models. Geographic sampling of the data, where samples of cynomolgus macaques derived from Indochina were excluded, revealed a lost signature of gene flow, indicating that interspecific gene flow is restricted to mainland Indochina. Our results, in conjunction with those by others, justify future detailed analyses into the genetics of reproductive barriers and reticulate evolution in these two genome-enabled primates. Future studies of the natural hybridization between rhesus and cynomolgus macaques would expand the repertoire of systems available for speciation studies in primates.