DIFFERENTIAL CELLULAR-REACTIVITY TO ADULT WORM ANTIGENS OF PATIENTS WITH DIFFERENT CLINICAL FORMS OF SCHISTOSOMIASIS-MANSONI
DIFFERENTIAL CELLULAR-REACTIVITY TO ADULT WORM ANTIGENS OF PATIENTS WITH DIFFERENT CLINICAL FORMS OF SCHISTOSOMIASIS-MANSONI
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DOI：
10.1016/0035-9203(92)90441-e
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发表时间：
1992-01-01
影响因子：
2.2
通讯作者：
CORREAOLIVEIRA, R
中科院分区：
文献类型：
--
作者：
BAHIAOLIVEIRA, LMG;GAZZINELLI, G;CORREAOLIVEIRA, R
使用总和分级可溶性成虫抗原 (SWAP) 对感染曼氏血吸虫并表现出不同临床形式的疾病的个体以及来自流行区的正常推定免疫个体的外周血单核细胞增殖和干扰素-γ (IFN-γ) 的产生进行体外分析。在患有较严重疾病的个体中，在 T 细胞蛋白质印迹测定中观察到对分级抗原的可检测反应频率更高。方差分析表明，在蛋白质印迹分析中，肝脾患者与肠道和肝肠疾病患者之间针对低分子量（< 21 kDa）抗原的细胞增殖水平存在统计学显着差异。没有观察到细胞增殖和IFN-γ产生之间的相关性。来自流行区的大多数正常个体未能对 SWAP T 细胞蛋白质印迹分析或固定在硝酸纤维素上的抗原表现出显着的增殖反应；它们确实对全可溶性 SWAP 表现出显着的增殖反应，并产生积极的 IFN-γ。结果与以下假设一致：对 SWAP 的细胞免疫反应的变化影响曼氏血吸虫病的病理发展和对感染的抵抗力。
Analysis of the proliferation in vitro of peripheral blood mononuclear cells and the production of interferon-gamma (IFN-gamma) in individuals infected with Schistosoma mansoni and showing different clinical forms of the disease, as well as normal putatively immune individuals from an endemic area, was undertaken using total and fractionated soluble adult worm antigens (SWAP). A higher frequency of detectable response to fractionated antigens in T cell Western blot assays was observed in individuals with the more severe forms of the disease. Analysis of variance showed that, in the Western blot assays, there was a statistically significant difference in the level of cellular proliferation to antigens with low molecular weight (< 21 kDa) between hepatosplenic patients and those with intestinal and hepatointestinal forms of the disease. No correlation between cellular proliferation and IFN-gamma production was observed. Most of the normal individuals from an endemic area failed to show significant proliferative responses to SWAP T cell Western blot assays or to antigen immobilized on nitrocellulose; they did show significant proliferative responses to whole soluble SWAP with positive IFN-gamma production. The results are consistent with the hypothesis that variations in the cellular immune responses to SWAP influence both the development of pathology and resistance to infection in schistosomiasis mansoni.