A COMMON MOTIF OF 2 ADJACENT PHOSPHOSERINES IN BOVINE, RABBIT AND HUMAN CARDIAC TROPONIN-I
A COMMON MOTIF OF 2 ADJACENT PHOSPHOSERINES IN BOVINE, RABBIT AND HUMAN CARDIAC TROPONIN-I
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DOI：
10.1016/0014-5793(90)81046-q
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发表时间：
1990-10-29
期刊：
影响因子：
3.5
通讯作者：
HEILMEYER, LMG
中科院分区：
文献类型：
--
作者：
MITTMANN, K;JAQUET, K;HEILMEYER, LMG
从兔和人心肌肌钙蛋白I中分离N-末端单磷酸化和双磷酸化肽，这些肽从Lys-C蛋白酶酶中获得。两个相邻的磷酸丝氨酸残基可以定位在每个磷酸肽进一步胰蛋白酶消化。先前发表的兔心肌肌钙蛋白I的序列必须予以纠正。两个相邻的磷酸丝氨酸残基是牛、兔和人心肌肌钙蛋白I非常相似的序列中的共同基序。N末端序列为：AcADRSGGSTAG DTVPAPPPVR RR ANYRAY ATEPHAK（牛）、AcADESTDA‐AG EARPAPVR RRANYRAY ATEPHAK（兔）、（Ac，A，D/N，G，S，S，D/N，A，A，R）EPRPAPIR RR‐NYRAY ATEPHAK（人）。
From rabbit and human cardiac troponin I N‐terminal mono and bisphosphorylated peptides were isolated which were obtained from Lys‐C proteinase digests. Two adjacent phosphoserine residues could be localized in each phosphopeptide following further tryptic digestion. The previously published sequence of rabbit cardiac troponin I had to be corrected. Two adjacent phosphoserine residues are a common motif in the very similar sequences of bovine, rabbit and human cardiac troponin I. The N‐terminal sequences are: AcADRSGGSTAG DTVPAPPPVR RR ANYRAY ATEPHAK (bovine), AcADESTDA‐AG EARPAPAPVR RRANYRAY ATEPHAK (rabbit), (Ac,A,D/N,G,S,S,D/N,A,A,R) EPRPAPAPIR RR‐NYRAY ATEPHAK (human).