Characterisation of major peptides in 'jack jumper' ant venom by mass spectrometry
Characterisation of major peptides in 'jack jumper' ant venom by mass spectrometry
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DOI：
10.1016/j.toxicon.2003.11.021
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发表时间：
2004-02-01
期刊：
影响因子：
2.8
通讯作者：
Browne, SGA
中科院分区：
文献类型：
--
作者：
Davies, NW;Wiese, MD;Browne, SGA
跳杰克蚁（Myrmannapilosula）是澳大利亚东南部的特有种，约有2.7%的人口对其毒液有全身过敏反应（过敏反应）史。以前的研究表明，Myr p 1基因的cDNA中含有几种变应原性肽，其中主要的变应原性表达产物为56个氨基酸残基的多肽（Myr p 157 -> 112，pilosulin 1，类似于6052 Da）。另一种主要的过敏原被描述为来自cDNA Myr p 2的27个残基的肽（Myr p 2 49 -> 75，'pilosulin 2'，类似于3212 Da），可能作为二硫键复合物的一部分存在。作为用于毒液免疫疗法的药物产品的详细稳定性研究的初步步骤，对通过电刺激和毒囊解剖从不同位置收集的毒液进行LC-MS和Edman测序分析。在LC-MS分析中检测到超过50种在4-9 kDa范围内的肽。发现Myr p 2的一个亚序列作为所有样品中存在的主要肽的一部分;这是由Myrp 249 -> 74组成的双-二硫键连接的、反平行排列的异二聚体，（脱-Gly（27）- pilosulin 2，类似于3155 Da）通过串联质谱和Edman序列测定，发现了一个与2457 Da相似的未报道的肽，主要是，有时是唯一的，作为以前未报道的类似于6067 Da的变体，其中残基5处的缬氨酸被异亮氨酸取代。在部分降解的毒液中也检测到[Ile（5）] pilosulin 1和pilosulin 1的一系列水解产物。进一步的IgE结合研究，使用这些肽是必要的，并修订命名的过敏性组分的M。可能需要pilosula毒液符合已建立的IUIS指南。(C)2004 Elsevier Ltd.保留所有权利。
The jack jumper ant, Myrmecia pilosula, is endemic to South-Eastern Australia, where around 2.7% of the population has a history of systemic allergic reactions (anaphylaxis) to its venom. Previous work had indicated that there were several allergenic peptides derived from the cDNA Myr p 1, the major expressed allergenic product being a 56-residue peptide (Myr p 157 --> 112, 'pilosulin 1', similar to6052 Da). Another major allergen had been described as a 27 residue peptide derived from the cDNA Myr p 2 (Myr p 2 49 --> 75, 'pilosulin 2', similar to3212 Da), possibly existing as part of a disulfide complex. As a preliminary step in detailed stability studies of a pharmaceutical product used for venom immunotherapy, LC-MS and Edman sequencing analysis of venom collected from various locations by both electrical stimulation and venom sac dissection was undertaken. More than 50 peptides in the 4-9 kDa range were detected in LC-MS analyses. A subsequence of Myr p 2 was found as part of the major peptide present in all samples; this was a bis-disulphide linked, antiparallel aligned heterodimer consisting of Myr p 249 --> 74, (des-Gly(27)- pilosulin 2, similar to3155 Da) and a previously unreported peptide of similar to2457 Da. Pilosulin 1 was found by a combination of tandem mass spectrometry and Edman sequencing to exist mainly, and sometimes exclusively, as a previously unreported similar to6067 Da variant, in which the valine at residue 5 is replaced by isoleucine. A range of hydrolysis products of [Ile(5)] pilosulin 1 and pilosulin 1 were also detected in partially degraded venom. Further IgE-binding studies using these peptides are warranted and a revision of the nomenclature of allergenic components of M. pilosula venom may be required to conform with established IUIS guidelines. (C) 2004 Elsevier Ltd. All rights reserved.