Quantification of macrophage content in atherosclerotic plaques by optical coherence tomography
Quantification of macrophage content in atherosclerotic plaques by optical coherence tomography
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DOI：
10.1161/01.cir.0000044384.41037.43
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发表时间：
2003-01-07
期刊：
影响因子：
37.8
通讯作者：
Bouma, BE
中科院分区：
文献类型：
--
作者：
Tearney, GJ;Yabushita, H;Bouma, BE
背景-巨噬细胞降解纤维帽基质是动脉粥样硬化斑块不稳定性的重要因素。能够识别患者体内巨噬细胞的成像技术可以为评估斑块脆弱性提供有价值的信息。光学相干断层扫描（OCT）是一种新的血管内成像方式，它可以对组织进行分辨率约为10 μ m的横截面成像。本研究的目的是探讨使用光学相干断层扫描识别巨噬细胞在纤维caps.Methods和Results-OCT图像的26个富含脂质的动脉粥样硬化动脉段在尸检中获得的组织学相关。通过用CD 68和平滑肌肌动蛋白进行免疫过氧化物酶染色，对帽巨噬细胞密度进行形态定量，并与相应位置的OCT信号强度的标准差进行比较。OCT与纤维帽巨噬细胞密度的组织学测量之间存在高度正相关（r=0.84，P
Background-Macrophage degradation of fibrous cap matrix is an important contributor to atherosclerotic plaque instability. An imaging technology capable of identifying macrophages in patients could provide valuable information for assessing plaque vulnerability. Optical coherence tomography (OCT) is a new intravascular imaging modality that allows cross-sectional imaging of tissue with a resolution of approximate to10 mum. The aim of this study was to investigate the use of OCT for identifying macrophages in fibrous caps.Methods and Results-OCT images of 26 lipid-rich atherosclerotic arterial segments obtained at autopsy were correlated with histology. Cap macrophage density was quantified morphometrically by immunoperoxidase staining with CD68 and smooth muscle actin and compared with the standard deviation of the OCT signal intensity at corresponding locations. There was a high degree of positive correlation between OCT and histological measurements of fibrous cap macrophage density (r=0.84, P