Isolation and characterization of wheat aluminum-regulated genes: possible involvement of aluminum as a pathogenesis response elicitor
Isolation and characterization of wheat aluminum-regulated genes: possible involvement of aluminum as a pathogenesis response elicitor
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DOI：
10.1007/s004250050352
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发表时间：
1998-08-01
期刊：
影响因子：
4.3
通讯作者：
Houde, M
中科院分区：
文献类型：
--
作者：
Hamel, F;Breton, C;Houde, M
利用差异筛选技术，从耐AI的小麦品种（Triticum aestivum L，cv. Atlas-66）暴露于铝，我们已经分离和表征了几个小麦铝调节（战争）的cDNA。序列比较显示，Al上调的基因对应于过氧化物酶（war 4，2）、半胱氨酸蛋白酶（war 5.2）、苯丙氨酸氨裂解酶（war 7.2）和草酸氧化酶（war 13，2）。两个小麦品种在他们的耐受性水平不同（CV。阿特拉斯-66：宽容，和CV。弗雷德里克：敏感）来评估War-mRNA的积累与Al毒性之间的关系，如通过根生长抑制（RGI）所测量的。mRNA的积累被调制到类似的水平，在两个品种相比，在同等的RGIs。这表明War mRNA的积累与铝的毒性有关，而不是与品种的耐受性有关。似乎大多数被Al上调的基因与病原体诱导的基因具有同源性。这表明，铝可能作为一个诱导剂的发病相关的转导途径。对上调的WAR基因在细胞壁强化和铝捕获中的潜在功能进行了讨论。
Using differential screening of a root tip cDNA library prepared from an AI-tolerant wheat cultivar (Triticum aestivum L, cv. Atlas-66) exposed to Al, we have isolated and characterized several wheat aluminum-regulated (War) cDNAs. Sequence comparison revealed that genes up-regulated by Al correspond to peroxidase (war4,2), cysteine proteinase (war 5.2), phenylalanine-ammonia lyase (war 7.2), and oxalate oxidase (war13,2). Two wheat cultivars that differ in their level of tolerance (cv. Atlas-66: tolerant, and cv. Fredrick: sensitive) were used to evaluate the relationship between the accumulation of War- mRNAs and Al toxicity, as measured by root growth inhibition (RGI). The mRNA accumulation was modulated to similar levels in both cultivars compared at equivalent RGIs. This indicates that War mRNA accumulation is associated with the toxicity of Al rather than with the cultivar's tolerance. It appears that most of the genes found to be up-regulated by Al share hamologies with genes induced by pathogens. This suggests that Al may act as an elicitor of a pathogenesis-related transduction pathway. The potential functions of the up-regulated war genes in cell wall strengthening and Al trapping are discussed.