Differentiated Caco-2 cell models in food-intestine interaction study: Current applications and future trends
Differentiated Caco-2 cell models in food-intestine interaction study: Current applications and future trends
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DOI：
10.1016/j.tifs.2020.11.015
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发表时间：
2021-01-01
影响因子：
15.3
通讯作者：
Yu, Qiang
中科院分区：
文献类型：
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作者：
Ding, Xiaomeng;Hu, Xiaoyi;Yu, Qiang
背景：由于肠道具有多种生理功能，食物与肠道相互作用的研究一直是食品科学和营养学领域的热门话题。与筛选能力有限的昂贵动物模型相比，简单、可靠、重复性高的肠细胞模型被广泛应用于食物-肠道相互作用研究。用于体外模拟肠道的功能细胞模型有多种，其中Caco-2细胞模型是应用最广泛、最经典的模型之一。近年来，由于各种技术的发展，分化的Caco-2细胞模型得到了很大的发展，不仅克服了传统模型的局限性，而且进一步拓宽了其应用范围。范围和方法：本文旨在概述分化的Caco-2细胞模型作为体外肠道细胞的专门模型的当前应用，以及解决现有应用挑战的新方法，以指导其与肠道细胞相互作用的未来趋势。 主要发现和结论：分化的Caco-2细胞模型具有高灵活性、高重复性和低成本，已应用于多种肠道研究，包括肠道吸收、肠道转运、肠道代谢、肠道屏障、肠道免疫和肠道粘附。此外，未来的研究应借助自动化、生物化学、分子生物学和细胞共培养等技术，突破传统模型的局限性，使其在不牺牲简单性和可靠性的情况下更接近内部环境，更适合经济有效的大规模食物-肠道相互作用分析。
Background: Food-intestine interaction study has always been a hot topic in food science and nutrition due to diverse physiological functions of intestine. Compared to expensive animal models with limited screening capabilities, the simple, reliable and highly reproducible intestinal cell models are widely used in food-intestine interaction study. There are many functional cell models used to simulate the intestine in vitro, among which the Caco-2 cell model is one of the most widely used and classical models. Recently years, the differentiated Caco-2 cell model has been greatly developed due to the development of various technologies, which not only overcomes the limitations of the traditional model, but also further broadens its application.Scope and approach: This review aims to overview the current applications of the differentiated Caco-2 cell model as a specialized model of intestinal cells in vitro, as well as new approaches solving the existing challenges of utilization, which can guide its future trends in interaction between food factors and the intestine.Key findings and conclusions: With high flexibility, high repeatability and low cost, the differentiated Caco-2 cell model has been applied to a variety of intestinal studies including intestinal absorption, intestinal transport, intestinal metabolism, intestinal barrier, intestinal immunity and intestinal adhesion. Furthermore, future study should break limitations of traditional models with the help of automation, biochemistry, molecular biology and cells co-culture, so as to make it more closer to the internal environment without sacrificing its simplicity and reliability, and more suitable for cost-effective large-scale analysis of food-intestine interaction.