Peptide derived from Pvfp-1 as bioadhesive on bio-inert surface
Peptide derived from Pvfp-1 as bioadhesive on bio-inert surface
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源自 Pvfp-1 的肽作为生物惰性表面上的生物粘合剂。
DOI：
10.1016/j.colsurfb.2011.10.037
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发表时间：
2012-02-01
影响因子：
5.8
通讯作者：
Zhang, Qiqing
中科院分区：
文献类型：
--
作者：
Jiang, Zhen;Yu, Yabiao;Zhang, Qiqing
表面性质是材料的一个重要特性，特别是对于那些生物惰性但设计用于垃圾医疗应用的材料。本研究设计了一系列多肽，并比较了它们作为生物粘附剂的能力，以提高生物惰性材料的表面生物活性。根据佩尔纳足蛋白1（Perna viridis foot protein 1，Pvfp-1）的序列设计肽段，Pvfp-1是贻贝足蛋白1（mussel foot protein 1，Mfp-1）的一种，在贻贝足丝的湿粘附中起关键作用。选择聚四氟乙烯（PTFE）作为生物惰性材料的模型。通过吸附、粘附和涂层分析，发现肽C2（M）（来自Pvfp-1的非重复区域，含有修饰的DOPA）具有上级的涂层和粘附能力，特别是在PTFE的生物惰性表面上。经C2（M）肽包被后，成骨细胞MC 3 T3-E1在PTFE表面的黏附和铺展能力明显增强，且肽包被对细胞无毒性。因此。肽C2（M）可有效地提高生物惰性PTFE的生物活性，并且可潜在地用作生物粘附剂在生物医学应用中的其他生物惰性材料上。此外，该研究也为设计其他基于肽的生物粘附材料提供了新的见解。(C)2011爱思唯尔有限公司版权所有。
Surface property is one important characteristic of materials, especially for ones that are bio-inert but designed for bin-medical application. In this study, we designed a series of peptides and compared their capacities as bioadhesive to improve the surface bioactivity of bio-inert material. The peptides were designed according to the sequence of Perna viridis foot protein 1 (Pvfp-1), one of the Mfp-1s (mussel foot protein 1) which play key roles in wet adhesion of mussel byssus. And the Teflon (PTFE) was chosen as a model of bio-inert material. With adsorption, adhesion and coating analysis, it was found that peptide C2 (M) (derived from the non-repeating region of Pvfp-1, contains modified DOPA) has superior coating and adhesion abilities especially on the bio-inert surface of PTFE. After coating with peptide C2 (M), the cell adhesion and spreading of osteoblast MC3T3-E1 cells on PTFE were significantly improved compared with those on non-coated surface, and the peptide-coating did not show any cell toxicity. Therefore. peptide C2 (M) is effective for improving the bioactivity of bio-inert PTFE, and could be potentially used as a bioadhesive on other bin-inert materials for biomedical application. Moreover, this study also provided new insights in designing other peptide-based bioadhesive materials. (C) 2011 Elsevier B.V. All rights reserved.