Single-cell Sequencing in the Field of Stem Cells.
Single-cell Sequencing in the Field of Stem Cells.
复制标题
干细胞领域的单细胞测序
DOI：
10.2174/1389202921999200624154445
复制
发表时间：
2020-12
期刊：
影响因子：
2.6
通讯作者：
Rong R
中科院分区：
文献类型：
--
作者：
Chen T;Li J;Jia Y;Wang J;Sang R;Zhang Y;Rong R
细胞间的差异性和异质性是干细胞的基本特征。传统的测序分析方法往往掩盖了这种差异。单细胞测序技术是指在单细胞水平上对基因组进行高通量测序分析的技术。它可以有效地分析细胞的异质性，并识别少量的细胞群体。随着细胞分选、核酸提取等技术的不断进步，单细胞测序技术也取得了长足的进步。干细胞研究取得了令人鼓舞的新发现，包括多能干细胞、组织特异性干细胞和癌症干细胞。本文就干细胞领域中单细胞测序技术的最新进展和发展前景作一综述。
Variation and heterogeneity between cells are the basic characteristics of stem cells. Traditional sequencing analysis methods often cover up this difference. Single-cell sequencing technology refers to the technology of high-throughput sequencing analysis of genomes at the single-cell level. It can effectively analyze cell heterogeneity and identify a small number of cell populations. With the continuous progress of cell sorting, nucleic acid extraction and other technologies, single-cell sequencing technology has also made great progress. Encouraging new discoveries have been made in stem cell research, including pluripotent stem cells, tissue-specific stem cells and cancer stem cells. In this review, we discuss the latest progress and future prospects of single-cell sequencing technology in the field of stem cells.