INHIBITION OF CONNECTIVE-TISSUE FORMATION IN DERMAL WOUNDS COVERED WITH SYNTHETIC, MOISTURE VAPOR PERMEABLE DRESSINGS AND ITS REVERSAL BY TRANSFORMING GROWTH-FACTOR-BETA
INHIBITION OF CONNECTIVE-TISSUE FORMATION IN DERMAL WOUNDS COVERED WITH SYNTHETIC, MOISTURE VAPOR PERMEABLE DRESSINGS AND ITS REVERSAL BY TRANSFORMING GROWTH-FACTOR-BETA
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DOI：
10.1111/1523-1747.ep12477982
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发表时间：
1990-08-01
影响因子：
6.5
通讯作者：
MCPHERSON, JM
中科院分区：
文献类型：
--
作者：
KSANDER, GA;PRATT, BM;MCPHERSON, JM
通过次要目的对合成、粘附性、透湿性敷料（SAM）在皮肤伤口愈合方面的研究产生了新的观察结果，即与小鼠和豚鼠系统中的空气暴露伤口相比，SAM敷料严重抑制肉芽组织和随后的胶原组织沉积。在覆盖SAM或暴露于空气中长达3周的全层伤口中，对修复组织进行组织形态计量学定量。在愈合早期，小鼠伤口暴露于大气中形成结痂，其覆盖来自两个来源的大量肉芽组织，一个来源于侧面，另一个来源于深部和中心位置。相比之下，SAM覆盖的伤口仅含有少量的肉芽组织，其仅来自侧面来源。随着时间的推移，肉芽组织被纤维结缔组织取代，在SAM覆盖的伤口中，这种情况总是较少。暴露于空气的伤口中大量结缔组织的沉积与显著的多形性和单核细胞浸润相关，而SAM覆盖部位缺乏肉芽组织形成与炎症减少相关。敷料诱导的结缔组织抑制可通过用转化生长因子β 1或2处理而部分逆转。当伤口覆盖SAM时，豚鼠皮肤中的大晶状体伤口中的肉芽组织沉积也受到抑制，但在6 mm的穿孔伤口中没有，并且暴露于空气和SAM覆盖的伤口的形态与小鼠相似。SAM覆盖的伤口在小鼠和豚鼠可能是有用的慢性不愈合伤口的模型。
An investigation of synthetic, adherent, moisture vapor-permeable dressings (SAM) on dermal wounds healing by secondary intent has yielded the novel observation that SAM dressings severely inhibited the deposition of granulation tissue and subsequent collagenous tissue when compared with air-exposed wounds in mouse and guinea pig systems. Repair tissue was quantitated histomorphometrically in full-thickness wounds covered with SAM or left air-exposed for periods up to 3 weeks. Early in healing, mouse wounds left open to the atmosphere formed a scab which overlay a large volume of granulation tissue derived from two sources, one lateral, and the other deep and centrally located. In contrast, SAM-covered wounds contained only a small amount of granulation tissue which was derived solely from lateral sources. Granulation tissue was replaced by fibrous connective tissue over time, and this was always less in SAM-covered wounds. Deposition of large amounts of connective tissue in air-exposed wounds was associated with significant polymorphonuclear and mononuclear cell infiltrates, while the lack of granulation tissue formation in SAM-covered sites was associated with reduced inflammation. Dressing-induced inhibition of connective tissue could be partially reversed by treatment with transforming growth factor-beta from 1 or 2. Deposition of granulation tissue in large lenticular wounds in guinea pig skin, but not in 6-mm punch wounds, was also inhibited when the wounds were covered with SAM, and the morphology of air-exposed and SAM-covered wounds was similar to that in mice. SAM-covered wounds in mice and guinea pigs may be useful as models of chronic non-healing wounds.