The Escherichia coli small heat shock protein IbpA plays a role in regulating the heat shock response by controlling the translation of σ32
The Escherichia coli small heat shock protein IbpA plays a role in regulating the heat shock response by controlling the translation of σ32
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大肠杆菌小热激蛋白IbpA通过控制σ32的翻译来发挥调节热激反应的作用
DOI：
10.1101/2023.03.28.534623
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Hideki Taguchi
中科院分区：
文献类型：
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作者：
Tsukumi Miwa; Hideki Taguchi
热休克蛋白IbpA在转录后水平调节其自身及其副产物ibpB的表达（28）。ibpA和ibpB mRNA的5′非翻译区存在的茎环构成了一个关键的调控元件。虽然在ibpA和ibpB的5′ UTR中常见具有多个茎环的二级结构，但它们的序列和结构并不相同（26，27）。因此，我们推测IbpA除了自身调节外，还可能影响大肠杆菌中其他蛋白的表达。杆菌为了确定IbpA介导的调节的潜在靶点，我们进行了基于质谱（MS）的定量蛋白质组学分析，以鉴定在E. coli中表达。我们比较了过表达IbpA的细胞的蛋白质组和作为对照的过表达GFP的细胞的蛋白质组，发现在IbpA过表达时，41和40种蛋白质分别特异性增加（> 1.67倍）和减少（< 0.6倍）（图1A和数据集S1）。基因本体论（GO）分析显示，在IbpA过表达中最显著改变和统计学显著的类别，
Heat Shock Proteins. IbpA regulates the expression of itself and its paralog, ibpB, at the posttranscriptional level (28). The stem loops present in the 5′ UTR of the mRNA of ibpAor ibpBconstitute a key regulatory element. While secondary structures with multiple stemloops are common in the 5′ UTRs of ibpA and ibpB, their sequences and structures are not identical (26, 27). Therefore, we hypothesized that, apart from self-regulation, IbpA may also influence the expression of other proteins in E. coli. To identify potential targets of IbpA-mediated regulation, we conducted a mass spectrometry (MS)–based quantitative proteomics analysis to identify proteins, whose expression is altered in E. coli upon IbpA overexpression. We compared the proteomes of cells overexpressing IbpA and those overexpressing GFP as a control and found that 41 and 40 proteins were specifically increased (> 1.67-fold) and decreased (< 0.6-fold), respectively, upon IbpA overexpression (Fig. 1A and Dataset S1). Gene ontology (GO) analysis revealed that the most significantly altered and statistically significant category in IbpA-overexpressed