Virus detection using nanoelectromechanical devices
Virus detection using nanoelectromechanical devices
复制标题
DOI：
10.1063/1.1794378
复制
发表时间：
2004-09-27
影响因子：
4
通讯作者：
Craighead, HG
中科院分区：
文献类型：
--
作者：
Ilic, B;Yang, Y;Craighead, HG
我们已经使用了一个共振机械悬臂检测免疫特异性结合的病毒，从液体中捕获。作为模型病毒，我们使用非致病性昆虫杆状病毒来测试特异性结合和检测少量病毒颗粒的能力。阵列的表面微加工，抗体涂覆的多晶硅纳米机械悬臂梁被用来检测从缓冲溶液中的杆状病毒的各种浓度的结合。由于它们的小质量，0.5 μ m × 6 μ m的杠杆具有10（-19）g/Hz数量级的质量灵敏度，使得能够检测对应于约3 × 10（-15）g质量的固定化的AcV 1抗体单层。有了这些设备，我们可以检测到结合在悬臂上的单个病毒颗粒的质量。由病毒颗粒的吸附质量引起的共振频移区分了病毒浓度在10（5）和10（7）pfu/ml之间变化的溶液。使用不含杆状病毒的缓冲液进行的对照实验显示，
We have used a resonating mechanical cantilever to detect immunospecific binding of viruses, captured from liquid. As a model virus, we used a nonpathogenic insect baculovirus to test the ability to specifically bind and detect small numbers of virus particles. Arrays of surface micromachined, antibody-coated polycrystalline silicon nanomechanical cantilever beams were used to detect binding from various concentrations of baculoviruses in a buffer solution. Because of their small mass, the 0.5 mumx6 mum cantilevers have mass sensitivities on the order of 10(-19) g/Hz, enabling the detection of an immobilized AcV1 antibody monolayer corresponding to a mass of about 3x10(-15) g. With these devices, we can detect the mass of single-virus particles bound to the cantilever. Resonant frequency shift resulting from the adsorbed mass of the virus particles distinguished solutions of virus concentrations varying between 10(5) and 10(7) pfu/ml. Control experiments using buffer solutions without baculovirus showed small amounts (