Mechanism of Mesoporous Silica Nanoparticle Interaction with Hairy Root Cultures during Nanoharvesting of Biomolecules.
Mechanism of Mesoporous Silica Nanoparticle Interaction with Hairy Root Cultures during Nanoharvesting of Biomolecules.
复制标题
生物分子纳米收获过程中介孔二氧化硅纳米粒子与毛状根培养物相互作用的机制。
DOI：
10.1002/adbi.202000173
复制
发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Knutson,BarbaraL
中科院分区：
文献类型：
--
作者：
Khan,MdArif;Fugate,Madeleine;Rogers,DennisT;Sambi,Jatinder;Littleton,JohnM;Rankin,StephenE;Knutson,BarbaraL
工程化介孔二氧化硅纳米颗粒（MSNP）的细胞摄取和排出机制在其用于新型生物分子分离和递送应用（例如纳米收获）的设计中是重要的，纳米收获被定义为使用纳米载体从活植物器官培养物（例如，毛状根）。在这里，毛状根中的温度依赖性MSNP吸收和恢复过程被检查为表面化学的函数。分别使用Ti含量（存在于生物分子结合中）和荧光标记的MSNP的荧光光谱定量MSNP摄取到毛状根中和时间依赖性排出。结果表明，功能化和表面电荷（由胺基连接调节）在吸收和恢复的有效性中起着最大的作用。在4 ° C和23 °C下MSNP与毛状根的相互作用的比较表明，仅用Ti官能化的弱电荷MSNP通过热活化机制被吸收和排出，而胺修饰的带正电荷的颗粒主要通过直接穿透细胞壁被吸收和排出。胺官能化的MSNP通过动态交换自发地进出植物细胞，停留时间为20 ± 5分钟，这表明有望作为植物器官培养的生物分子纳米收获平台。
Cellular uptake and expulsion mechanisms of engineered mesoporous silica nanoparticles (MSNPs) are important in their design for novel biomolecule isolation and delivery applications such as nanoharvesting, defined as using nanocarriers to transport and isolate valuable therapeutics (secondary metabolites) out of living plant organ cultures (e.g., hairy roots). Here, temperature‐dependent MSNP uptake and recovery processes in hairy roots are examined as a function of surface chemistry. MSNP uptake into hairy roots and time‐dependent expulsion are quantified using Ti content (present for biomolecule binding) and fluorescence spectroscopy of fluorescently tagged MSNPs, respectively. The results suggest that functionalization and surface charge (regulated by amine group attachment) play the biggest role in the effectiveness of uptake and recovery. Comparison of MSNP interactions with hairy roots at 4 and 23 °C shows that weakly charged MSNPs functionalized only with Ti are taken up and expelled by thermally activated mechanisms, while amine‐modified positively charged particles are taken up and expelled mainly by direct penetration of cell walls. Amine‐functionalized MSNPs move spontaneously in and out of plant cells by dynamic exchange with a residence time of 20 ± 5 min, suggesting promise as a biomolecule nanoharvesting platform for plant organ cultures.