MECHANISMS OF LIPID PEROXIDE FORMATION IN ANIMAL TISSUES
MECHANISMS OF LIPID PEROXIDE FORMATION IN ANIMAL TISSUES
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DOI：
10.1042/bj0990667
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发表时间：
1966-01-01
影响因子：
4.1
通讯作者：
WILLS, ED
中科院分区：
文献类型：
--
作者：
WILLS, ED
1.大鼠肝脏、脾脏、心脏和肾脏的匀浆在体外孵育时形成脂质过氧化物，并在亚油酸或亚麻酸乳剂中积极催化过氧化物的形成。2.在肝脏中，催化活性分布在整个细胞核、线粒体和微粒体组分中，并存在于100000 g上清液中。核部分的活动很弱。3.稀释（0.5%，w/v）匀浆在pH 5.0 - 8.0范围内催化过氧化反应，但浓缩（5%，w/v）匀浆抑制过氧化反应，并在溶液碱性高于pH 7.0时破坏过氧化物。4.在pH6.0时，抗坏血酸可增加肝、心、肾和脾全匀浆催化的不饱和脂肪酸的过氧化速率，但在pH7.4时不增加。5.在pH7.4时，抗坏血酸可抑制肝脏线粒体和微粒体组分对不饱和脂肪酸过氧化反应的催化作用，但其上清组分的活性增强。6.无机铁或铁蛋白在抗坏血酸存在下是活性催化剂。7.组织匀浆催化的亚油酸或亚麻酸乳剂中脂质过氧化物的形成被EDTA部分抑制，但被邻菲咯啉刺激。8.半胱氨酸或谷胱甘肽（1 mm）抑制整个匀浆、线粒体或血蛋白催化的过氧化物形成。抑制作用随pH值的增加而增加。
1. Homogenates of rat liver, spleen, heart and kidney form lipid peroxides when incubated in vitro and actively catalyse peroxide formation in emulsions of linoleic acid or linolenic acid. 2. In liver, catalytic activity is distributed throughout the nuclear, mitochondrial and microsomal fractions and is present in the 100000g supernatant. Activity is weak in the nuclear fraction. 3. Dilute (0·5%, w/v) homogenates catalyse peroxidation over the range pH5·0–8·0 but concentrated (5%, w/v) homogenates inhibit peroxidation and destroy peroxide if the solution is more alkaline than pH7·0. 4. Ascorbic acid increases the rate of peroxidation of unsaturated fatty acids catalysed by whole homogenates of liver, heart, kidney and spleen at pH6·0 but not at pH7·4. 5. Catalysis of peroxidation of unsaturated fatty acids by the mitochondrial and microsomal fractions of liver is inhibited by ascorbic acid at pH7·4 but the activity of the supernatant fraction is enhanced. 6. Inorganic iron or ferritin are active catalysts in the presence of ascorbic acid. 7. Lipid peroxide formation in linoleic acid or linolenic acid emulsions catalysed by tissue homogenates is partially inhibited by EDTA but stimulated by o-phenanthroline. 8. Cysteine or glutathione (1mm) inhibits peroxide formation catalysed by whole homogenates, mitochondria or haemoprotein. Inhibition increases with increase of pH.