The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
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DOI：
10.1111/j.1365-313x.2011.04691.x
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发表时间：
2011-10-01
期刊：
影响因子：
7.2
通讯作者：
Ferreira, Paulo C. G.
中科院分区：
文献类型：
--
作者：
Eloy, Nubia B.;Lima, Marcelo de Freitas;Ferreira, Paulo C. G.
最大的E3泛素-连接酶复合物，称为后期促进复合物/细胞周期体（APC/C），调节细胞周期调节因子如细胞周期蛋白B和细胞周期蛋白的蛋白水解，其对于姐妹染色单体分离和退出有丝分裂是必需的。尽管其重要性，APC/C在植物细胞中的作用及其在细胞分裂过程中的活性调节仍然知之甚少。在这里，拟南芥APC/C亚基APC 10的特点，并显示出功能互补的apc 10酵母突变体。APC 10蛋白位于特定的核小体中，最可能是由于其与蛋白酶体复合物的结合。一个apc 10拟南芥敲除突变体强烈损害女性配子发生。令人惊讶的是，APC 10的组成型过表达增强了叶的大小。通过运动学分析，增加的叶片大小被发现是由于在叶片发育的早期阶段细胞分裂的速率增强，并在分子水平上，通过增加APC/C活性，如有丝分裂细胞周期蛋白，CYCB 1的蛋白水解率的放大测量;1。
The largest E3 ubiquitin-ligase complex, known as anaphase-promoting complex/cyclosome (APC/C), regulates the proteolysis of cell cycle regulators such as CYCLIN B and SECURIN that are essential for sister-chromatid separation and exit from mitosis. Despite its importance, the role of APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, the Arabidopsis thaliana APC/C subunit APC10 was characterized and shown to functionally complement an apc10 yeast mutant. The APC10 protein was located in specific nuclear bodies, most probably resulting from its association with the proteasome complex. An apc10 Arabidopsis knockout mutant strongly impaired female gametogenesis. Surprisingly, constitutive overexpression of APC10 enhanced leaf size. Through kinematic analysis, the increased leaf size was found to be due to enhanced rates of cell division during the early stages of leaf development and, at the molecular level, by increased APC/C activity as measured by an amplification of the proteolysis rate of the mitotic cyclin, CYCB1;1.