Identification procedure for Pasteurella pneumotropica in microbiologic monitoring of laboratory animals
Identification procedure for Pasteurella pneumotropica in microbiologic monitoring of laboratory animals
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DOI：
10.1538/expanim.54.123
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发表时间：
2005-04-01
影响因子：
2.4
通讯作者：
Itoh, T
中科院分区：
文献类型：
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作者：
Hayashimoto, N;Aiba, T;Itoh, T
已认识到检测实验室之间嗜肺巴氏杆菌的鉴定存在差异。为了确定差异的原因并提出嗜肺假单胞菌的可靠鉴定程序，组织了一个工作组，从日本的8个实验室收集了69株鉴定或疑似嗜肺假单胞菌的分离株。采用菌落形态学、革兰氏染色、玻片凝集试验（抗ATCC 35149和MaR）、两种市售生化试剂盒（ID test，AP 120 NE）和两套PCR引物（Wang PCR，CIEA PCR）对这些分离株进行了检测。根据检测结果将69株分离株和2株参考菌株分为10组。没有发现单一的嗜肺假单胞菌鉴定方法，在供试菌株中，除了菌落颜色取决于其生物型外，菌落形态和革兰氏染色没有观察到大的差异。69个分离株中有68个通过使用两种抗血清的玻片凝集试验呈阳性，除了一个分离株用一种抗血清进行检测。ID试验鉴定了69株分离株中的61株为嗜肺假单胞菌，与ClEA PCR的结果没有大的差异。根据这些结果，我们建议将菌落观察、革兰氏染色、两种抗血清的玻片凝集试验和ID试验的生化试验相结合，以实用和可靠地鉴定该微生物。
Discrepancies have been recognized in the identification of Pasteurella pneumotropica between testing laboratories. To determine the causes of the differences and to propose a reliable identification procedure for P. pneumotropica, a working group was organized and 69 isolates identified or suspected as P. pneumotropica were collected from 8 laboratories in Japan. These isolates were examined by colony morphology, Gramstaining, the slide agglutination test using two antisera (ATCC35149 and MaR), two commercially available biochemical test kits (ID test, AP120NE) and two primer sets of PCR tests (Wang PCR, CIEA PCR). The 69 isolates and two reference strains were divided into 10 groups by test results. No single procedure for P. pneumotropica identification was found. Among tested isolates, large differences were not observed by colony morphology and Gram-straining except for colony colors that depended on their biotypes. Sixty-eight out of 69 isolates were positive by the slide agglutination test using two antisera except for one isolate that tested with one antiserum. The ID test identified 61 out of 69 isolates as P. pneumotropica and there was no large difference from the results of ClEA PCR. From these results, we recommend the combination of colony observation, Gram-straining, the slide agglutination tests with two antisera and biochemical test using the ID test for practical and reliable identification of this organism.