Fabrication and characterization of lignin-xylan hybrid nanospheres as pesticide carriers with enzyme-mediated release property
Fabrication and characterization of lignin-xylan hybrid nanospheres as pesticide carriers with enzyme-mediated release property
复制标题
具有酶介导释放特性的木质素-木聚糖杂化纳米球作为农药载体的制备和表征
DOI：
10.1039/d0sm01402h
复制
发表时间：
2020-10-21
期刊：
影响因子：
3.4
通讯作者：
Shen, Fei
中科院分区：
文献类型：
--
作者：
Jiang, Yuehan;Chen, Yiyi;Shen, Fei
木质素纳米微球是实现木质素增值的新兴高价值材料平台。对LNP进行修饰或引入新的功能对拓展其下游应用具有重要意义。本研究以木质素-木聚糖杂化纳米微球（LXNPs）为载体，通过简单的溶液自组装技术制备了LXNPs，并将其应用于农药的酶介导控释。使用深共熔溶剂提取的缩合木质素和水不溶性木聚糖片段获得具有各种重量比（木质素与木聚糖，3：1、1：1、1：3）的杂化LXNP，其表现出约166-210 nm的纳米球尺寸，在4-10的pH范围内具有相当大的稳定性。木质素与木聚糖的比例为3：1和1：1的LXNP显示出明确的核-壳结构，在表面上具有富集的羟基。这表明木质素通过货车范德华力和木质素苯丙烷与木聚糖分子骨架之间的疏水相互作用，可以锚木聚糖片段，从而促进了这种特殊球形结构形成的自组装过程。所得疏水性LXNP核使得能够使用一锅法合成以57.9-67.0%的效率容易地包封生物农药阿维菌素（AVM）。当这些AVM包封的LXNP进行酶水解使用木聚糖酶，相当大的AVM释放44.8-55.1%，16小时后实现，相比之下，只有4.1%的释放没有木聚糖酶。这项工作显示了通过自组装过程制造混合LXNPs的高前景，并且还为药物包封和随后的酶介导的控制释放提供了通用的纳米球载体。
Lignin nanospheres (LNPs) are an emerging high-value material platform to realize lignin valorization. The modification or introduction of new functions to LNPs is of great significance to expand its downstream applications. This work evaluated the technical feasibility of preparing lignin-xylan hybrid nanospheres (LXNPs) through a simple solution-based self-assembly process, with the goal of achieving the application as pesticide carriers for enzyme-mediated controlled release. Hybrid LXNPs with various weigh ratios (lignin to xylan, 3 : 1, 1 : 1, 1 : 3) were obtained using deep eutectic solvent-extracted condensed lignin and water-insoluble xylan fragments, which exhibited a nanosphere size of about 166-210 nm with considerable stability in the pH range of 4-10. LXNPs with lignin to xylan ratios of 3 : 1 and 1 : 1 showed well-defined core-shell structures with enriched hydroxyl groups on the surface. It was proposed that lignin could anchor xylan fragments through van der Waals force and hydrophobic interactions between lignin phenylpropanes and xylan molecular backbones, thus facilitating the self-assembly process for the formation of this specific spherical structure. The resulting hydrophobic LXNPs core enabled the facile encapsulation of the biological pesticide avermectin (AVM) with 57.9-67.0% efficiency using one-pot synthesis. When these AVM-encapsulated LXNPs were subjected to enzymatic hydrolysis using xylanase, considerable AVM release of 44.8-55.1% was achieved after 16 h, in comparison to the 4.1% release only for those without xylanase. This work showed the high promise of fabricating hybrid LXNPs through the self-assembly process and also provided a universal nanosphere carrier for drug encapsulation and subsequent enzyme-mediated controlled release.