The Dynein Stalk Contains an Antiparallel Coiled Coil with Region-Specific Stability
The Dynein Stalk Contains an Antiparallel Coiled Coil with Region-Specific Stability
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DOI：
10.1021/bi900223x
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发表时间：
2009-03-31
期刊：
影响因子：
2.9
通讯作者：
Vallee, Richard B.
中科院分区：
文献类型：
--
作者：
Hook, Peter;Yagi, Toshiki;Vallee, Richard B.
动力蛋白马达蛋白与不寻常的 12-15 nm 茎远端的微管相互作用，微管以循环、双向的方式与核苷酸水解和微管结合的位点进行通信。在这里，我们报道大鼠细胞质动力蛋白的茎轴是一个反平行的α螺旋卷曲线圈，其稳定性因其近端和远端的变化而显着改变，这与动力蛋白跨桥循环期间能够快速、周期性重排的结构一致。
The dynein motor proteins interact with microtubules at the distal end of an unusual 12-15 nm stalk, which communicates with the sites for nucleotide hydrolysis and microtubule binding in a cyclical, bidirectional manner. Here, we report that the stalk shaft of rat cytoplasmic dynein is an antiparallel a-helical coiled coil, the stability of which is markedly altered by changes at its proximal and distal ends, consistent with a structure capable of rapid, cyclical rearrangement during the dynein cross-bridge cycle.