An Integrated Approach Exploring the Synergistic Mechanism of Herbal Pairs in a Botanical Dietary Supplement: A Case Study of a Liver Protection Health Food
An Integrated Approach Exploring the Synergistic Mechanism of Herbal Pairs in a Botanical Dietary Supplement: A Case Study of a Liver Protection Health Food
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DOI：
10.1155/2020/9054192
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发表时间：
2020-04-11
影响因子：
2.9
通讯作者：
Du, Jun
中科院分区：
文献类型：
--
作者：
Chen, Liang;Hu, Chun;Du, Jun
药对是连接单味药与复方的桥梁，为复杂的方剂提供理论基础。中药对的有效性和合理性已被广泛应用作为膳食补充剂的策略。然而，由于植物化学的复杂性，个别和组合的草药材料，这是很难揭示其有效的和协同作用的机制，从分子或系统的角度来看。为了解决这一问题，UPLC-Q-TOF/MS分析和系统药理学工具被应用于探索作用机制，使用基于白色牡丹（白芍）和甘草（甘草）的膳食补充剂。从白色牡丹和甘草中分离鉴定了16种化学成分，它们与73个保肝相关靶点相互作用。然后沿着网络分析进行基因本体和京都基因和基因组百科全书（KEGG）途径富集分析。结果表明，白色牡丹和甘草药对的协同作用机制与其协同调节胆汁分泌和ABC转运途径有关。此外，甘草对药物和异源生物质代谢途径表现出特异性反应，而白色牡丹对Toll样受体信号传导、C型凝集素受体信号传导、IL-17信号传导和TNF信号传导途径有反应，从而预防肝细胞凋亡并减少免疫和炎症介导的肝损伤。这些研究结果表明，白色牡丹和甘草草药对补充将有一个肝脏保护的好处，通过互补和协同机制。该方法为从中医理论出发探索中药在膳食补充剂中的配伍提供了一条新的途径。
Herbal pairs are used as a bridge between single herb and polyherbal formulas in Traditional Chinese Medicine (TCM) to provide rationale for complicated TCM formulas. The effectiveness and rationality of TCM herbal pairs have been widely applied as a strategy for dietary supplements. However, due to the complexity of the phytochemistry of individual and combinations of herbal materials, it is difficult to reveal their effective and synergistic mechanisms from a molecular or systematic point of view. In order to address this question, UPLC-Q-TOF/MS analysis and System Pharmacology tools were applied to explore the mechanism of action, using a White Peony (Paeoniae Radix Alba) and Licorice (Glycyrrhizae Radix et Rhizoma)-based dietary supplement. A total of sixteen chemical constituents of White Peony and Licorice were isolated and identified, which interact with 73 liver protection-related targets. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were then performed along with network analysis. Results showed that the synergistic mechanism of the White Peony and Licorice herbal pair was associated with their coregulation of bile secretion and ABC transporter pathways. In addition, Licorice exhibits a specific response to drug and xenobiotic metabolism pathways, whereas White Peony responds to Toll-like receptor signaling, C-type lectin receptor signaling, IL-17 signaling, and TNF signaling pathways, resulting in the prevention of hepatocyte apoptosis and the reduction of immune and inflammation-mediated liver damage. These findings suggest that a White Peony and Licorice herbal pair supplement would have a liver-protecting benefit through complimentary and synergistic mechanisms. This approach provides a new path to explore herbal compatibility in dietary supplements derived from TCM theory.