Differential miRNA expression in pleural effusions derived from extracellular vesicles of patients with lung cancer, pulmonary tuberculosis, or pneumonia
Differential miRNA expression in pleural effusions derived from extracellular vesicles of patients with lung cancer, pulmonary tuberculosis, or pneumonia
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肺癌、肺结核或肺炎患者细胞外囊泡胸腔积液中差异 miRNA 表达
DOI：
10.1007/s13277-016-5410-6
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发表时间：
2016-12-01
期刊：
影响因子：
--
通讯作者：
Wang, Xiao-Zhong
中科院分区：
文献类型：
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作者：
Lin, Jin;Wang, Yan;Wang, Xiao-Zhong
MicroRNAs （miRNAs）在各种生理和病理过程中发挥着重要的调节作用。mirna也表现出高稳定性，并以高浓度存在于人体体液中。因此，mirna可能代表某些临床条件有吸引力和新颖的诊断生物标志物。最近，研究人员描述了细胞外囊泡（包括微囊泡和外泌体）携带参与细胞间通讯的mirna的能力。本研究采用高通量测序技术检测了肺炎（A组）、肺结核（B组）和肺癌（C组）患者三种胸腔积液的miRNA表达谱。比较三组之间这些mirna的表达水平，A组和B组之间检测到3个差异mirna， A组和C组之间检测到27个差异mirna。其中，只有B组miR-378i显著升高，而只有C组miR-205-5p和miR-200b显著升高（p< 0.01）。需要进一步的研究来证实这些差异表达的mirna是否可以作为肺部疾病的前瞻性诊断标志物。
MicroRNAs (miRNAs) have been found to play important regulatory roles in various physiological and pathological processes. MiRNAs also exhibit high stability and are present at high concentrations in human bodily fluids. Consequently, miRNAs may represent attractive and novel diagnostic biomarkers for certain clinical conditions. Recently, the capacity for extracellular vesicles, including microvesicles and exosomes, to carry miRNAs that participate in cell-to-cell communication has been described. In the present study, the miRNA expression patterns for three kinds of pleural effusions that were obtained from patients with pneumonia (group A), pulmonary tuberculosis (group B), and lung cancer (group C) were detected with high-throughput sequencing. When the expression levels of these miRNAs were compared among the three groups, three differentially expressed miRNAs were detected between groups A and B, while 27 differentially expressed miRNAs were detected between groups A and C. Notably, miR-378i was significantly elevated only in group B, while miR-205-5p and miR-200b were markedly increased only in group C (p< 0.01). Further studies are needed to confirm whether these differentially expressed miRNAs may serve as prospective diagnostic markers for pulmonary diseases.