Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice.
Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice.
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小鼠心脏外植体细胞辐射损伤的剂量依赖性和可逆性
DOI：
10.1038/srep40959
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发表时间：
2017-01-18
影响因子：
4.6
通讯作者：
Li TS
中科院分区：
文献类型：
--
作者：
Luo L;Yan C;Urata Y;Hasan AS;Goto S;Guo CY;Zhang S;Li TS
我们评估了辐射诱导的心肌细胞（CDCs）损伤的剂量依赖性和可逆性，CDCs是从心脏组织中生长的混合细胞群。成年C57 BL/6小鼠分别接受0、10、50和250 mGy γ射线照射7 d，末次照射后24 h取心房组织进行实验。每日照射量超过250 mGy时，CDC的数量显著减少。每日照射剂量超过50 mGy后，CDCs中c-kit表达和端粒酶活性明显降低，53 BP 1灶明显增多。然而，CD 90表达和生长因子的生产在CDCs没有显着改变，即使每天暴露于250毫戈瑞。我们进一步评估了单次暴露于3戈伊γ射线后1周和3周CDCs辐射诱导损伤的可逆性。1周后，CDCs的数量和生长因子的产生很快恢复。而CD 90的表达增加在1周时仍保持，但在3周时恢复。此外，即使在3周时，c-kit表达降低、端粒酶活性受损和53 BP 1灶增加也恢复不良。这些数据可能有助于我们找到最敏感和可靠的生物学参数来评估放射性损伤的CDC。
We evaluated the dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells (CDCs), a mixed cell population grown from heart tissues. Adult C57BL/6 mice were exposed to 0, 10, 50 and 250 mGy γ-rays for 7 days and atrial tissues were collected for experiments 24 hours after last exposure. The number of CDCs was significantly decreased by daily exposure to over 250 mGy. Interestingly, daily exposure to over 50 mGy significantly decreased the c-kit expression and telomerase activity, increased 53BP1 foci in the nuclei of CDCs. However, CD90 expression and growth factors production in CDCs were not significantly changed even after daily exposure to 250 mGy. We further evaluated the reversibility of radiation-induced injury in CDCs at 1 week and 3 weeks after a single exposure to 3 Gy γ-rays. The number and growth factors production of CDCs were soon recovered at 1 week. However, the increased expression of CD90 were retained at 1 week, but recovered at 3 weeks. Moreover, the decreased expression of c-kit, impaired telomerase activity, and increased 53BP1 foci were poorly recovered even at 3 weeks. These data may help us to find the most sensitive and reliable bio-parameter(s) for evaluating radiation-induced injury in CDCs.