MARKERS OF NEOPLASTIC TRANSFORMATION IN EPITHELIAL-CELL LINES DERIVED FROM HUMAN CARCINOMAS
MARKERS OF NEOPLASTIC TRANSFORMATION IN EPITHELIAL-CELL LINES DERIVED FROM HUMAN CARCINOMAS
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DOI：
10.1093/jnci/58.6.1743
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发表时间：
1977-01-01
影响因子：
10.3
通讯作者：
CARBONELL, AW
中科院分区：
文献类型：
--
作者：
MARSHALL, CJ;FRANKS, LM;CARBONELL, AW
在上皮肿瘤细胞系中测定了密度依赖性生长抑制、3T3[小鼠成纤维细胞]融合单层上的涂膜效率和琼脂中的生长，以确定它们是否与转化的间充质细胞具有共同的特性。5个系（RT4、RT112、J82、T24和EJ）来源于不同的人膀胱肿瘤，HT29来源于人结肠肿瘤。在没有密度依赖性生长抑制和细胞表面大的外部转化敏感蛋白的情况下，所有细胞系与转化成纤维细胞相似，它们可以在3T3单层上形成菌落。只有RT112、EJ和HT29在裸鼠中具有致瘤性，尽管细胞经过多次体外传代，但肿瘤具有许多原始肿瘤和组织或来源的结构和超微结构特征。其中4个细胞系（J82、T24、EJ、HT29）在琼脂中生长，因此在一些细胞系中发现琼脂生长与裸鼠致瘤性没有相关性：J82和T24在琼脂中生长，而致瘤性细胞系RT112没有在琼脂中生长。在琼脂中生长的能力似乎与高水平的纤溶酶原激活剂的产生无关。
Density dependent inhibition of growth, plating efficiency on confluent monolayers of 3T3 [mouse fibroblast] cells and growth in agar were measured in epithelial tumor cell lines to determine whether they have properties in common with transformed mesenchymal cells. Five lines (RT4, RT112, J82, T24 and EJ) were derived from different human bladder tumors and HT29 was from a human colon tumor. All lines resembled transformed fibroblasts in the absence of density dependent inhibition of growth and the cell surface large external transformation-sensitive protein, and they could form colonies on 3T3 monolayers. Only RT112, EJ and HT29 were tumorigenic in nude mice, and the tumors had many of the structural and ultrastructural features of the original tumor and tissue or origin, even though the cells were through many in vitro passages. Four of the lines (J82, T24, EJ, HT29) grew in agar, so that in some cell lines no correlation of growth in agar with tumorigenicity in nude mice was found: J82 and T24 were nontumorigenic and grew in agar, whereas tumorigenic line RT112 did not grow in agar. The ability to grow in agar did not appear associated the prouction of high levels of plasminogen activator.